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Saudi J Biol Sci. 2013 Jul;20(3):235-9. doi: 10.1016/j.sjbs.2013.02.003. Epub 2013 Feb 20.

Free radical scavenging activity, total phenolic content, total antioxidant status, and total oxidant status of endemic Thermopsis turcica.

Saudi journal of biological sciences

Laçine Aksoy, Erdi Kolay, Yasin Ağılönü, Zeyneb Aslan, Mustafa Kargıoğlu

Affiliations

  1. Department of Chemistry (Biochemistry Division), Faculty of Science and Arts, Afyon Kocatepe University, Afyonkarahisar 03200, Turkey.

PMID: 23961240 PMCID: PMC3730902 DOI: 10.1016/j.sjbs.2013.02.003

Abstract

Thermopsis turcica, endemic to Turkey, is in danger of extinction. Studies on this species are very few due to the fact that it was only discovered in 1983 and grows in a small circumscribed area in Turkey. In this study, free radical scavenging activity, total phenolic content, total oxidant status (TOS), and total antioxidant status (TAS) of methanol (TTM) and acetone (TTA) extracts of T. turcica were measured spectroscopically. Free radical scavenging activity was determined according to the elimination of DPPH radicals and total phenol content was determined by the Folin-Ciocalteu reaction. Total oxidant status (TOS) and total antioxidant status (TAS) were measured with commercially available kits. Methanol and acetone extracts of T. turcica were found to have a specific radical scavenging effect. This effect was found to be related to the total phenolic content of the extracts. Since the TTA had a higher phenolic content than the methanol extract, it had a stronger radical scavenging effect. In addition, the total antioxidant capacity of the methanol extract was observed to be higher than that of its acetone counterpart. As a result, due to its antioxidative properties, T. turcica is thought to be a natural source of antioxidants.

Keywords: DPPH; Radical scavenging activity; TAS; TOS; Thermopsis turcica; Total phenolic content

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