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Gitlin L, Hoera C, Meier RJ, et al. Micro flow reactor chips with integrated luminescent chemosensors for spatially resolved on-line chemical reaction monitoring. Lab Chip. 2013;13(20):4134-41doi: 10.1039/c3lc50387a.
Gitlin, L., Hoera, C., Meier, R. J., Nagl, S., & Belder, D. (2013). Micro flow reactor chips with integrated luminescent chemosensors for spatially resolved on-line chemical reaction monitoring. Lab on a chip, 13(20), 4134-41. https://doi.org/10.1039/c3lc50387a
Gitlin, Leonid, et al. "Micro flow reactor chips with integrated luminescent chemosensors for spatially resolved on-line chemical reaction monitoring." Lab on a chip vol. 13,20 (2013): 4134-41. doi: https://doi.org/10.1039/c3lc50387a
Gitlin L, Hoera C, Meier RJ, Nagl S, Belder D. Micro flow reactor chips with integrated luminescent chemosensors for spatially resolved on-line chemical reaction monitoring. Lab Chip. 2013 Oct 21;13(20):4134-41. doi: 10.1039/c3lc50387a. Epub 2013 Aug 23. PMID: 23970303.
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Lab Chip. 2013 Oct 21;13(20):4134-41. doi: 10.1039/c3lc50387a. Epub 2013 Aug 23.

Micro flow reactor chips with integrated luminescent chemosensors for spatially resolved on-line chemical reaction monitoring.

Lab on a chip

Leonid Gitlin, Christian Hoera, Robert J Meier, Stefan Nagl, Detlev Belder

Affiliations

  1. Institut für Analytische Chemie, Universität Leipzig, Johannisallee 29, 04103 Leipzig, Germany. [email protected].

PMID: 23970303 DOI: 10.1039/c3lc50387a

Abstract

Real-time chemical reaction monitoring in microfluidic environments is demonstrated using luminescent chemical sensors integrated in PDMS/glass-based microscale reactors. A fabrication procedure is presented that allows for straightforward integration of thin polymer layers with optical sensing functionality in microchannels of glass-PDMS chips of only 150 μm width and of 10 to 35 μm height. Sensor layers consisting of polystyrene and an oxygen-sensitive platinum porphyrin probe with film thicknesses of about 0.5 to 4 μm were generated by combining spin coating and abrasion techniques. Optimal coating procedures were developed and evaluated. The chip-integrated sensor layers were calibrated and investigated with respect to stability, reproducibility and response times. These microchips allowed observation of dissolved oxygen concentration in the range of 0 to over 40 mg L(-1) with a detection limit of 368 μg L(-1). The sensor layers were then used for observation of a model reaction, the oxidation of sulphite to sulphate in a microfluidic chemical reactor and could observe sulphite concentrations of less than 200 μM. Real-time on-line monitoring of this chemical reaction was realized at a fluorescence microscope setup with 405 nm LED excitation and CCD camera detection.

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