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Gaetti-Jardim Júnior E, Nakano V, Wahasugui TC, et al. Occurrence of yeasts, enterococci and other enteric bacteria in subgingival biofilm of HIV-positive patients with chronic gingivitis and necrotizing periodontitis. Braz J Microbiol. 2008;39(2):257-61doi: 10.1590/S1517-838220080002000011.
Gaetti-Jardim Júnior, E., Nakano, V., Wahasugui, T. C., Cabral, F. C., Gamba, R., & Avila-Campos, M. J. (2008). Occurrence of yeasts, enterococci and other enteric bacteria in subgingival biofilm of HIV-positive patients with chronic gingivitis and necrotizing periodontitis. Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 39(2), 257-61. https://doi.org/10.1590/S1517-838220080002000011
Gaetti-Jardim Júnior, Elerson, et al. "Occurrence of yeasts, enterococci and other enteric bacteria in subgingival biofilm of HIV-positive patients with chronic gingivitis and necrotizing periodontitis." Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] vol. 39,2 (2008): 257-61. doi: https://doi.org/10.1590/S1517-838220080002000011
Gaetti-Jardim Júnior E, Nakano V, Wahasugui TC, Cabral FC, Gamba R, Avila-Campos MJ. Occurrence of yeasts, enterococci and other enteric bacteria in subgingival biofilm of HIV-positive patients with chronic gingivitis and necrotizing periodontitis. Braz J Microbiol. 2008 Apr;39(2):257-61. doi: 10.1590/S1517-838220080002000011. Epub 2008 Jun 01. PMID: 24031212; PMCID: PMC3768404.
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Braz J Microbiol. 2008 Apr;39(2):257-61. doi: 10.1590/S1517-838220080002000011. Epub 2008 Jun 01.

Occurrence of yeasts, enterococci and other enteric bacteria in subgingival biofilm of HIV-positive patients with chronic gingivitis and necrotizing periodontitis.

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]

Elerson Gaetti-Jardim Júnior, Viviane Nakano, Thais C Wahasugui, Fátima C Cabral, Rosa Gamba, Mario Julio Avila-Campos

Affiliations

  1. Laboratório de Patologia, Faculdade de Odontologia de Araçatuba, Universidade Estadual de São Paulo , Araçatuba, SP , Brasil.

PMID: 24031212 PMCID: PMC3768404 DOI: 10.1590/S1517-838220080002000011

Abstract

The purpose of this study was to determine the prevalence of enteric bacteria and yeasts in biofilm of 80 HIV-positive patients with plaque-associated gingivitis or necrotizing periodontitis. Patients were subjected to extra, intra oral and radiographic examinations. The oral hygiene, bleeding on probing, gingival conditions, and attachment loss were evaluated. Clinical specimens were collected from gingival crevices or periodontal pockets, transferred to VMGA III, diluted and transferred to Sabouraud Dextrose agar with 100 μg/ml of chloramphenicol, peptone water, EVA broth, EMB agar, SS agar, Bile esculin agar and Brilliant green agar. Isolation of yeasts was carried out at room temperature, for 3-7 days; and for the isolation of enteric microorganisms plates were incubated at 37°C, for 24-48 h. The yeasts identification was performed according to the carbon and nitrogen assimilation, fermentation of carbohydrates and germ tube formation. Bacteria were identified according to their colonial and cellular morphologies and biochemical tests. Yeasts were identified as Candida albicans and its occurrence was more common in patients with CD4+ below 200/mm(3) and was affected by the extension of periodontal involvement (P = 0.0345). Enteric bacteria recovered from clinical specimens were identified as Enterobacter sakazakii, Enterobacter cloacae, Serratia liquefaciens, Klebsiella oxytoca and Enterococcus sp. Enterobacteriaceae and enterococci were detected in 32.5% of clinical samples from patients with necrotizing periodontitis. In conclusion, non-oral pathogenic bacteria and C. albicans were more prevalent in periodontal sites of HIV-positive patients with necrotizing periodontitis and chronic gingivitis.

Keywords: Candida albicans; HIV patients; enterobacteria; enterococci

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