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Boriollo MF, Spolidorio DM, Barros LM, et al. Typing Candida albicans oral isolates from healthy brazilian schoolchildren using multilocus enzyme electrophoresis reveals two highly polymorphic taxa. Braz J Microbiol. 2011;42(3):1030-46doi: 10.1590/S1517-838220110003000023.
Boriollo, M. F., Spolidorio, D. M., Barros, L. M., Bassi, R. C., Garcia, J. A., Costa, A. M., Rosa, E. A., & Höfling, J. F. (2011). Typing Candida albicans oral isolates from healthy brazilian schoolchildren using multilocus enzyme electrophoresis reveals two highly polymorphic taxa. Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 42(3), 1030-46. https://doi.org/10.1590/S1517-838220110003000023
Boriollo, Marcelo Fabiano Gomes, et al. "Typing Candida albicans oral isolates from healthy brazilian schoolchildren using multilocus enzyme electrophoresis reveals two highly polymorphic taxa." Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] vol. 42,3 (2011): 1030-46. doi: https://doi.org/10.1590/S1517-838220110003000023
Boriollo MF, Spolidorio DM, Barros LM, Bassi RC, Garcia JA, Costa AM, Rosa EA, Höfling JF. Typing Candida albicans oral isolates from healthy brazilian schoolchildren using multilocus enzyme electrophoresis reveals two highly polymorphic taxa. Braz J Microbiol. 2011 Jul;42(3):1030-46. doi: 10.1590/S1517-838220110003000023. Epub 2011 Sep 01. PMID: 24031720; PMCID: PMC3768784.
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Braz J Microbiol. 2011 Jul;42(3):1030-46. doi: 10.1590/S1517-838220110003000023. Epub 2011 Sep 01.

Typing Candida albicans oral isolates from healthy brazilian schoolchildren using multilocus enzyme electrophoresis reveals two highly polymorphic taxa.

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]

Marcelo Fabiano Gomes Boriollo, Denise Madalena Palomari Spolidorio, Letizia Monteiro Barros, Rodrigo Carlos Bassi, José Antonio Dias Garcia, Ana Maria Duarte Dias Costa, Edvaldo Antonio Ribeiro Rosa, José Francisco Höfling

Affiliations

  1. Laboratório de Genética e Biologia Molecular, Faculdade de Ciências Médicas, Universidade de Alfenas , Alfenas, MG , Brasil.

PMID: 24031720 PMCID: PMC3768784 DOI: 10.1590/S1517-838220110003000023

Abstract

The genetic diversity of C. albicans oral isolates from 75 healthy schoolchildren from eight schools located in different geographic areas of Piracicaba city, São Paulo state, Brazil, was established using isoenzymes marker (Multilocus Enzyme Electrophoresis - MLEE) and cluster analysis. Patterns of monoclonal and polyclonal oral colonization by C. albicans within and between groups of schoolchildren were identified. However, significant divergence between the observed and the expected genotypic frequencies (Hardy-Weinberg equilibrium test) was not detected in the geographically adjacent groups, suggesting the hypothesis that populations of healthy schoolchildren do not correspond to the selection factor (differential survival) of strains. Two highly polymorphic and distantly genetically related taxa (A and B) were identified within the total population of yeasts, each contained subgroups (A1, A2, A3, A4, B1 and B2) and clusters of moderately related strains (from I to X), suggesting the existence of strains restricted or not to certain groups of geographically limited, healthy students. However, the coexistence of identical strains in healthy schoolchildren from the same school (geographically related) reinforces the hypothesis of oral transmission, where the sources of propagation could be explored. Furthermore, this could also be used in current and retrospective analyses of C. albicans isolated from immunocompetent and immunocompromised people, in order to detect commensal or potentially pathogenic yeast groups, predominantly in candidiasis, and in the development of strategies to prevent transmission or human propagation.

Keywords: Candida albicans; MLEE; cluster analysis; geographic region; healthy schoolchildren

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