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Dos Santos EO, Michelon M, Furlong EB, et al. Evaluation of the composition of culture medium for yeast biomass production using raw glycerol from biodiesel synthesis. Braz J Microbiol. 2012;43(2):432-40doi: 10.1590/S1517-83822012000200002.
Dos Santos, E. O., Michelon, M., Furlong, E. B., Burkert, J. F., Kalil, S. J., & Burkert, C. A. (2012). Evaluation of the composition of culture medium for yeast biomass production using raw glycerol from biodiesel synthesis. Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 43(2), 432-40. https://doi.org/10.1590/S1517-83822012000200002
Dos Santos, Elisane Odriosolla, et al. "Evaluation of the composition of culture medium for yeast biomass production using raw glycerol from biodiesel synthesis." Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] vol. 43,2 (2012): 432-40. doi: https://doi.org/10.1590/S1517-83822012000200002
Dos Santos EO, Michelon M, Furlong EB, Burkert JF, Kalil SJ, Burkert CA. Evaluation of the composition of culture medium for yeast biomass production using raw glycerol from biodiesel synthesis. Braz J Microbiol. 2012 Apr;43(2):432-40. doi: 10.1590/S1517-83822012000200002. Epub 2012 Jun 01. PMID: 24031849; PMCID: PMC3768819.
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Braz J Microbiol. 2012 Apr;43(2):432-40. doi: 10.1590/S1517-83822012000200002. Epub 2012 Jun 01.

Evaluation of the composition of culture medium for yeast biomass production using raw glycerol from biodiesel synthesis.

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]

Elisane Odriosolla Dos Santos, Mariano Michelon, Eliana Badiale Furlong, Janaína Fernandes de Medeiros Burkert, Susana Juliano Kalil, Carlos André Veiga Burkert

Affiliations

  1. Universidade Federal do Rio Grande, Escola de Química e Alimentos, Laboratório de Engenharia de Bioprocessos Rio Grande, RS , Brasil.

PMID: 24031849 PMCID: PMC3768819 DOI: 10.1590/S1517-83822012000200002

Abstract

The work herewith investigated the production of yeast biomass as a source of protein, using Yarrowia lipolytica NRRL YB-423 and raw glycerol from biodiesel synthesis as the main carbon source. A significant influence of glycerol concentration, initial pH and yeast extract concentration on biomass and protein content was observed according to the 2v (5-1) fractional design. These factors were further evaluated using a central composite design and response surface methodology, and an empirical model for protein content was established and validated. The biomass of Yarrowia lipolytica NRRL YB-423 reached 19.5 ± 1.0 g/L in shaken flasks cultivation, with a protein content of 20.1 ± 0.6% (w/w).

Keywords: Raw glycerol; Yarrowia lipolytica; single cell protein; yeast biomass

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