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Li R, Jiang Y, Wang X, et al. Psychrotrophic Pseudomonas mandelii CBS-1 produces high levels of poly-β-hydroxybutyrate. Springerplus. 2013;2:335doi: 10.1186/2193-1801-2-335.
Li, R., Jiang, Y., Wang, X., Yang, J., Gao, Y., Zi, X., Zhang, X., Gao, H., & Hu, N. (2013). Psychrotrophic Pseudomonas mandelii CBS-1 produces high levels of poly-β-hydroxybutyrate. SpringerPlus, 2335. https://doi.org/10.1186/2193-1801-2-335
Li, Rongpeng, et al. "Psychrotrophic Pseudomonas mandelii CBS-1 produces high levels of poly-β-hydroxybutyrate." SpringerPlus vol. 2 (2013): 335. doi: https://doi.org/10.1186/2193-1801-2-335
Li R, Jiang Y, Wang X, Yang J, Gao Y, Zi X, Zhang X, Gao H, Hu N. Psychrotrophic Pseudomonas mandelii CBS-1 produces high levels of poly-β-hydroxybutyrate. Springerplus. 2013 Jul 23;2:335. doi: 10.1186/2193-1801-2-335. eCollection 2013. PMID: 24010027; PMCID: PMC3755782.
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Springerplus. 2013 Jul 23;2:335. doi: 10.1186/2193-1801-2-335. eCollection 2013.

Psychrotrophic Pseudomonas mandelii CBS-1 produces high levels of poly-β-hydroxybutyrate.

SpringerPlus

Rongpeng Li, Yuji Jiang, Xinfeng Wang, Jingjing Yang, Yuan Gao, Xiaoli Zi, Xia Zhang, Haofeng Gao, Nan Hu

Affiliations

  1. Colloge of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 211800 People's Republic of China.

PMID: 24010027 PMCID: PMC3755782 DOI: 10.1186/2193-1801-2-335

Abstract

A novel facultative psychrotroph (strain CBS-1), which accumulates poly-β-hydroxybutyrate (PHB), was isolated from soil samples taken from Changbai Mountain, China. Phylogenetic analysis based on 16S rRNA sequence data and Biolog analysis identified strain CBS-1 as Pseudomonas mandelii. Transmission electron micrographs revealed abundant electron-transparent intracellular granules. (1)H-nuclear magnetic resonance analysis revealed that the granules were composed of PHB. P. mandelii CBS-1 grew optimally at 20°C. When cultured aerobically for 48 h with sucrose as the sole carbon source, strain CBS-1 yielded a maximum cell density of 29.3 g/L cell dry weight and synthesized 22.3 g/L of PHB. The ability of strain CBS-1 to grow at a low temperature and rapidly synthesize high levels of PHB may reduce the costs of industrial PHB production.

Keywords: 1H- nuclear magnetic resonance; Facultative psychrotrophs; Fermentation; PHB; Pseudomonas mandelii CBS-1

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