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Chen CY, Chen CT. Reaction-based and single fluorescent emitter decorated ratiometric nanoprobe to detect hydrogen peroxide. Chemistry. 2013;19(47):16050-7doi: 10.1002/chem.201302342.
Chen, C. Y., & Chen, C. T. (2013). Reaction-based and single fluorescent emitter decorated ratiometric nanoprobe to detect hydrogen peroxide. Chemistry (Weinheim an der Bergstrasse, Germany), 19(47), 16050-7. https://doi.org/10.1002/chem.201302342
Chen, Chun-Yen, and Chen, Chao-Tsen. "Reaction-based and single fluorescent emitter decorated ratiometric nanoprobe to detect hydrogen peroxide." Chemistry (Weinheim an der Bergstrasse, Germany) vol. 19,47 (2013): 16050-7. doi: https://doi.org/10.1002/chem.201302342
Chen CY, Chen CT. Reaction-based and single fluorescent emitter decorated ratiometric nanoprobe to detect hydrogen peroxide. Chemistry. 2013 Nov 18;19(47):16050-7. doi: 10.1002/chem.201302342. Epub 2013 Oct 10. PMID: 24123627.
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Chemistry. 2013 Nov 18;19(47):16050-7. doi: 10.1002/chem.201302342. Epub 2013 Oct 10.

Reaction-based and single fluorescent emitter decorated ratiometric nanoprobe to detect hydrogen peroxide.

Chemistry (Weinheim an der Bergstrasse, Germany)

Chun-Yen Chen, Chao-Tsen Chen

Affiliations

  1. Department of Chemistry, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei, 10617 Taiwan (R.O.C.), Fax: (+886)?2-23636359.

PMID: 24123627 DOI: 10.1002/chem.201302342

Abstract

A novel reaction-based cross-linked polymeric nanoprobe with a self-calibrating ratiometric fluorescence readout to selectively detect H2O2 is reported. The polymeric nanoprobe is fabricated by using hydrophobic H2O2-reactive boronic ester groups, crosslinker units, and environmentally sensitive 3-hydroxyflavone fluorophores through a miniemulsion polymerization. On treatment with H2O2, the boronic esters in the polymer are cleaved to form hydrophilic alcohols and subsequently lead to a hydrophobic-hydrophilic transition. Covalently linked 3-hydroxyflavones manifest the change in polarity as a ratiometric transition from green to blue, accompanied by a 500-fold increase in volume. Furthermore, this nanoprobe has been used for ratiometric sensing of glucose by monitoring the H2O2 generated during the oxidation of glucose by glucose oxidase, and thus successfully distinguished between normal and pathological levels of glucose.

Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Keywords: fluorescent probes; hydrogen peroxide; nanoparticles; sensors; volume swelling

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