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Le Mével JC, Lancien F, Mimassi N, et al. Central ventilatory and cardiovascular actions of trout gastrin-releasing peptide (GRP) in the unanesthetized trout. Biol Open. 2013;2(9):960-7doi: 10.1242/bio.20135553.
Le Mével, J. C., Lancien, F., Mimassi, N., Kermorgant, M., & Conlon, J. M. (2013). Central ventilatory and cardiovascular actions of trout gastrin-releasing peptide (GRP) in the unanesthetized trout. Biology open, 2(9), 960-7. https://doi.org/10.1242/bio.20135553
Le Mével, Jean-Claude, et al. "Central ventilatory and cardiovascular actions of trout gastrin-releasing peptide (GRP) in the unanesthetized trout." Biology open vol. 2,9 (2013): 960-7. doi: https://doi.org/10.1242/bio.20135553
Le Mével JC, Lancien F, Mimassi N, Kermorgant M, Conlon JM. Central ventilatory and cardiovascular actions of trout gastrin-releasing peptide (GRP) in the unanesthetized trout. Biol Open. 2013 Jul 30;2(9):960-7. doi: 10.1242/bio.20135553. eCollection 2013. PMID: 24143283; PMCID: PMC3773343.
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Biol Open. 2013 Jul 30;2(9):960-7. doi: 10.1242/bio.20135553. eCollection 2013.

Central ventilatory and cardiovascular actions of trout gastrin-releasing peptide (GRP) in the unanesthetized trout.

Biology open

Jean-Claude Le Mével, Frédéric Lancien, Nagi Mimassi, Marc Kermorgant, J Michael Conlon

Affiliations

  1. Université Européenne de Bretagne, Université de Brest, INSERM UMR1101, Laboratoire de Traitement de l'Information Médicale, Laboratoire de Neurophysiologie, SFR ScInBioS, Faculté de Médecine et des Sciences de la Santé , 22 Avenue Camille Desmoulins, CS 93837, 29238 Brest Cedex 3, CHU de Brest , France.

PMID: 24143283 PMCID: PMC3773343 DOI: 10.1242/bio.20135553

Abstract

Gastrin-releasing peptide (GRP), a neuropeptide initially isolated from porcine stomach, shares sequence similarity with bombesin. GRP and its receptors are present in the brains and peripheral tissues of several species of teleost fish, but little is known about the ventilatory and cardiovascular effects of this peptide in these vertebrates. The goal of this study was to compare the central and peripheral actions of picomolar doses of trout GRP on ventilatory and cardiovascular variables in the unanesthetized rainbow trout. Compared to vehicle, intracerebroventricular (ICV) injection of GRP (1-50 pmol) significantly elevated the ventilation rate (ƒV) and the ventilation amplitude (VAMP), and consequently the total ventilation (VTOT). The maximum hyperventilatory effect of GRP (VTOT: +225%), observed at a dose of 50 pmol, was mostly due to its stimulatory action on VAMP (+170%) rather than ƒV (+20%). In addition, ICV GRP (50 pmol) produced a significant increase in mean dorsal aortic blood pressure (P DA) (+35%) and in heart rate (ƒH) (+25%). Intra-arterial injections of GRP (5-100 pmol) were without sustained effect on the ventilatory variables but produced sporadic and transient increases in ventilatory movement at doses of 50 and 100 pmol. At these doses, GRP elevated P DA by +20% but only the 50 pmol dose significantly increased HR (+15%). In conclusion, our study suggests that endogenous GRP within the brain of the trout may act as a potent neurotransmitter and/or neuromodulator in the regulation of cardio-ventilatory functions. In the periphery, endogenous GRP may act as locally-acting and/or circulating neurohormone with an involvement in vasoregulatory mechanisms.

Keywords: Central GRP; Hypertension; Hyperventilation; Intracerebroventricular injection; Teleost

References

  1. Brain Res. 1985 Aug 26;341(2):261-8 - PubMed
  2. Biochem Biophys Res Commun. 1979 Sep 12;90(1):227-33 - PubMed
  3. Ross Fiziol Zh Im I M Sechenova. 2012 May;98(5):598-609 - PubMed
  4. Regul Pept. 1995 Dec 14;60(2-3):167-76 - PubMed
  5. Biochem Biophys Res Commun. 1991 Jul 31;178(2):526-30 - PubMed
  6. Science. 1977 May 27;196(4293):998-1000 - PubMed
  7. Gen Comp Endocrinol. 1987 Dec;68(3):415-20 - PubMed
  8. J Anat. 1989 Aug;165:191-200 - PubMed
  9. Brain Res. 1993 Apr 30;610(1):82-9 - PubMed
  10. Comp Biochem Physiol C Comp Pharmacol Toxicol. 1988;89(2):249-56 - PubMed
  11. J Comp Neurol. 1990 Dec 22;302(4):893-919 - PubMed
  12. Regul Pept. 1991 Sep 3;35(3):207-19 - PubMed
  13. Eur J Pharmacol. 1983 Jun 17;90(4):449-51 - PubMed
  14. Gen Comp Endocrinol. 2009 Feb 1;160(3):250-8 - PubMed
  15. Brain Behav Evol. 1988;31(3):181-92 - PubMed
  16. Acta Physiol Scand. 1980 Jun;109(2):217-23 - PubMed
  17. Peptides. 2004 Mar;25(3):511-20 - PubMed
  18. Front Endocrinol (Lausanne). 2012 Oct 30;3:124 - PubMed
  19. Comp Biochem Physiol C Comp Pharmacol Toxicol. 1985;82(1):81-9 - PubMed
  20. J Exp Biol. 2007 Sep;210(Pt 18):3301-10 - PubMed
  21. Regul Pept. 1990 Sep 10;30(2):125-35 - PubMed
  22. Eur J Pharmacol. 2009 Jan 5;602(1):157-62 - PubMed
  23. Am J Physiol. 1994 May;266(5 Pt 2):R1568-77 - PubMed
  24. Eur J Pharmacol. 1985 Sep 10;115(1):21-9 - PubMed
  25. Peptides. 1992 Sep-Oct;13(5):995-9 - PubMed
  26. Experientia. 1971 Feb 15;27(2):166-7 - PubMed
  27. Front Endocrinol (Lausanne). 2012 Dec 17;3:159 - PubMed
  28. Peptides. 2000 May;21(5):639-48 - PubMed
  29. Proc Natl Acad Sci U S A. 1984 Sep;81(18):5699-703 - PubMed
  30. J Exp Biol. 1967 Apr;46(2):297-305 - PubMed
  31. Neuroscience. 2004;124(4):973-84 - PubMed
  32. Auton Neurosci. 2012 Aug 16;169(2):63-9 - PubMed
  33. Brain Res. 2004 Oct 15;1023(2):167-74 - PubMed
  34. J Comp Physiol B. 2011 Apr;181(3):311-33 - PubMed
  35. J Pharm Pharmacol. 1970 Nov;22(11):875-6 - PubMed
  36. Peptides. 1983 May-Jun;4(3):277-81 - PubMed
  37. Peptides. 1994;15(6):1027-32 - PubMed
  38. Gen Comp Endocrinol. 2009 Oct;164(1):51-60 - PubMed
  39. Pharmacol Rep. 2011;63(2):448-54 - PubMed
  40. Life Sci. 1977 Dec 15;21(12):1729-34 - PubMed

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