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Pfeil U, van der Kuip H, Hesemann CU. Non-radioactive organization and transcript analysis of the ATPase subunit 6 gene region in the mitochondrial genome from fertile and sterile (CMS) forms of wheat and triticale. Theor Appl Genet. 1994;88(2):231-5doi: 10.1007/BF00225902.
Pfeil, U., van der Kuip, H., & Hesemann, C. U. (1994). Non-radioactive organization and transcript analysis of the ATPase subunit 6 gene region in the mitochondrial genome from fertile and sterile (CMS) forms of wheat and triticale. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 88(2), 231-5. https://doi.org/10.1007/BF00225902
Pfeil, U, et al. "Non-radioactive organization and transcript analysis of the ATPase subunit 6 gene region in the mitochondrial genome from fertile and sterile (CMS) forms of wheat and triticale." TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik vol. 88,2 (1994): 231-5. doi: https://doi.org/10.1007/BF00225902
Pfeil U, van der Kuip H, Hesemann CU. Non-radioactive organization and transcript analysis of the ATPase subunit 6 gene region in the mitochondrial genome from fertile and sterile (CMS) forms of wheat and triticale. Theor Appl Genet. 1994 May;88(2):231-5. doi: 10.1007/BF00225902. PMID: 24185931.
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Theor Appl Genet. 1994 May;88(2):231-5. doi: 10.1007/BF00225902.

Non-radioactive organization and transcript analysis of the ATPase subunit 6 gene region in the mitochondrial genome from fertile and sterile (CMS) forms of wheat and triticale.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

U Pfeil, H van der Kuip, C U Hesemann

Affiliations

  1. Institute of Genetics, University of Hohenheim, D-70593, Stuttgart, Germany.

PMID: 24185931 DOI: 10.1007/BF00225902

Abstract

In sterile triticale forms [with cytoplasmic male-sterile (CMS)-inducing timopheevi cytoplasm], fertile orms (with normal cytoplasm) and the corresponding wheat cross parents mitochondrial DNA (mtDNA) and RNA (messenger mtRNA) were characterized using total DNA and RNA material for Southern and Northern blots. A novel non-radioactive technique was applied by marking the probes with digoxenin. The fertile and sterile Triticum and triticale forms were analysed in three genes, atp 6, coxIII and rps13. These forms can be distinguished in the apt6 gene at the mtDNA and mtRNA levels.

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