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Bajaj S, Rajam MV. Efficient plant regeneration from long-term callus cultures of rice by spermidine. Plant Cell Rep. 1995;14(11):717-20doi: 10.1007/BF00232654.
Bajaj, S., & Rajam, M. V. (1995). Efficient plant regeneration from long-term callus cultures of rice by spermidine. Plant cell reports, 14(11), 717-20. https://doi.org/10.1007/BF00232654
Bajaj, S, and Rajam, M V. "Efficient plant regeneration from long-term callus cultures of rice by spermidine." Plant cell reports vol. 14,11 (1995): 717-20. doi: https://doi.org/10.1007/BF00232654
Bajaj S, Rajam MV. Efficient plant regeneration from long-term callus cultures of rice by spermidine. Plant Cell Rep. 1995 Aug;14(11):717-20. doi: 10.1007/BF00232654. PMID: 24186629.
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Plant Cell Rep. 1995 Aug;14(11):717-20. doi: 10.1007/BF00232654.

Efficient plant regeneration from long-term callus cultures of rice by spermidine.

Plant cell reports

S Bajaj, M V Rajam

Affiliations

  1. Department of Genetics, University of Delhi, South Campus, Benito Juarez Road, -110021, New Delhi, India.

PMID: 24186629 DOI: 10.1007/BF00232654

Abstract

A significant reduction in regeneration potential with increasing age (upto 12months) in rice (Oryza sativa L. cv.TN-1) embryogenic callus cultures was observed. Spermidine, while having an inhibitory effect on plant regeneration in fresh callus cultures, promoted morphogenesis in long-term callus cultures. A massive accumulation of polyamines, particularly putrescine (5-fold) was observed in 12 month old cultures resulting in a change of putrescine /spermidine ratio, which seems to be important for maintaining the morphogenetic response. Application of exogenous spermidine to 12 month old cultures showed increased levels of polyamines and restored the putrescine/spermidine ratio comparable to that found in freshly induced cultures, concomitantly, promoting the plant regeneration via somatic embryogenesis in long-term rice callus cultures.

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