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Musgrave A, Kuin H, Jongen M, et al. Ethanol stimulates phospholipid turnover and inositol 1,4,5-trisphosphate production in Chlamydomonas eugametos gametes. Planta. 1992;186(3):442-9doi: 10.1007/BF00195326.
Musgrave, A., Kuin, H., Jongen, M., de Wildt, P., Schuring, F., Klerk, H., & van den Ende, H. (1992). Ethanol stimulates phospholipid turnover and inositol 1,4,5-trisphosphate production in Chlamydomonas eugametos gametes. Planta, 186(3), 442-9. https://doi.org/10.1007/BF00195326
Musgrave, A, et al. "Ethanol stimulates phospholipid turnover and inositol 1,4,5-trisphosphate production in Chlamydomonas eugametos gametes." Planta vol. 186,3 (1992): 442-9. doi: https://doi.org/10.1007/BF00195326
Musgrave A, Kuin H, Jongen M, de Wildt P, Schuring F, Klerk H, van den Ende H. Ethanol stimulates phospholipid turnover and inositol 1,4,5-trisphosphate production in Chlamydomonas eugametos gametes. Planta. 1992 Feb;186(3):442-9. doi: 10.1007/BF00195326. PMID: 24186742.
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Planta. 1992 Feb;186(3):442-9. doi: 10.1007/BF00195326.

Ethanol stimulates phospholipid turnover and inositol 1,4,5-trisphosphate production in Chlamydomonas eugametos gametes.

Planta

A Musgrave, H Kuin, M Jongen, P de Wildt, F Schuring, H Klerk, H van den Ende

Affiliations

  1. Department of Molecular Cell Biology, University of Amsterdam, Kruislaan 318, NL-1098, SM Amsterdam, The Netherlands.

PMID: 24186742 DOI: 10.1007/BF00195326

Abstract

Alcohols induce mating-structure activation in Chlamydomonas eugametos gametes. From the effect of ethanol on the (32)P-labelling of polyphosphoinositides, we conclude that the synthesis of these lipids is stimulated. Biologically inactive concentrations of ethanol (<6%) had no effect on synthesis, but 6-8% ethanol stimulated synthesis for upto 60 min. The (32)P incorporated into polyphosphoinositides and phosphatidic acid during ethanol treatment was readily chased out when 1 mM unlabelled Na3PO4 was added. Using a binding assay for inositol 1,4,5-trisphosphate, we show that the production of this phospholipid constituent is dramatically increased after ethanol treatment. This effect, coupled to a rise in intracellular calcium concentration, could explain gamete activation. The significance of these results in explaining other ethanol-induced phenomena in algae is discussed.

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