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Stöger E, Fink C, Pfosser M, et al. Plant transformation by particle bombardment of embryogenic pollen. Plant Cell Rep. 1995;14(5):273-8doi: 10.1007/BF00232027.
Stöger, E., Fink, C., Pfosser, M., & Heberle-Bors, E. (1995). Plant transformation by particle bombardment of embryogenic pollen. Plant cell reports, 14(5), 273-8. https://doi.org/10.1007/BF00232027
Stöger, E, et al. "Plant transformation by particle bombardment of embryogenic pollen." Plant cell reports vol. 14,5 (1995): 273-8. doi: https://doi.org/10.1007/BF00232027
Stöger E, Fink C, Pfosser M, Heberle-Bors E. Plant transformation by particle bombardment of embryogenic pollen. Plant Cell Rep. 1995 Feb;14(5):273-8. doi: 10.1007/BF00232027. PMID: 24186759.
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Plant Cell Rep. 1995 Feb;14(5):273-8. doi: 10.1007/BF00232027.

Plant transformation by particle bombardment of embryogenic pollen.

Plant cell reports

E Stöger, C Fink, M Pfosser, E Heberle-Bors

Affiliations

  1. Vienna Biocenter, Institute of Microbiology and Genetics, Dr. Bohrgasse 9, A-1030, Wien, Austria.

PMID: 24186759 DOI: 10.1007/BF00232027

Abstract

Direct delivery of DNA into embryogenic pollen was used to produce transgenic plants in tobacco. A plasmid bearing the ß-glucuronidase (GUS) marker gene in fusion with the 35S-promoter was introduced by microprojectile bombardment into mid-binucleate pollen of Nicotiana tabacum that had been induced to form embryos by a starvation treatment. In cytochemical expression assays, 5 out of 10(4) pollen grains were GUS(+). Visual selection by staining with a non-lethal substrate for GUS was used to manually isolate transformed embryos. From the initial population of embryogenic GUS(+) pollen, 1-5% developed into multicellular structures and 0.02% formed regenerable embryos. Two haploid transformants were regenerated. GUS expression was detected in different parts of the plants, and Southern analysis confirmed stable integration of the foreign DNA. Diploidisation was induced by injection of colchicine into the stem near adventitious buds. Offspring from selfings and backcrosses of one transformant were tested for GUS expression and by Southern blots. All F1-plants were transgenic, in accordance with Mendelian inheritance.

References

  1. Plant Cell Rep. 1989 Oct;8(7):387-90 - PubMed
  2. Plant Cell Rep. 1987 Apr;6(2):94-7 - PubMed
  3. Transgenic Res. 1992 Mar;1(2):63-70 - PubMed
  4. Plant Physiol. 1989 Dec;91(4):1270-4 - PubMed
  5. Mol Gen Genet. 1990 Jan;220(2):245-50 - PubMed
  6. Plant Cell Rep. 1990 Jul;9(2):69-72 - PubMed
  7. Plant Cell Rep. 1992 Feb;11(1):20-4 - PubMed
  8. Plant Cell Rep. 1991 Jun;10(2):55-8 - PubMed

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