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Boeer K, Cuznetov L, Loesche W. Thrombin generation as marker to estimate thrombosis risk in patients with abnormal test results in lupus anticoagulant routine diagnostics. Thromb J. 2013;11(1):24doi: 10.1186/1477-9560-11-24.
Boeer, K., Cuznetov, L., & Loesche, W. (2013). Thrombin generation as marker to estimate thrombosis risk in patients with abnormal test results in lupus anticoagulant routine diagnostics. Thrombosis journal, 11(1), 24. https://doi.org/10.1186/1477-9560-11-24
Boeer, Klas, et al. "Thrombin generation as marker to estimate thrombosis risk in patients with abnormal test results in lupus anticoagulant routine diagnostics." Thrombosis journal vol. 11,1 (2013): 24. doi: https://doi.org/10.1186/1477-9560-11-24
Boeer K, Cuznetov L, Loesche W. Thrombin generation as marker to estimate thrombosis risk in patients with abnormal test results in lupus anticoagulant routine diagnostics. Thromb J. 2013 Nov 12;11(1):24. doi: 10.1186/1477-9560-11-24. PMID: 24219775; PMCID: PMC3842625.
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Thromb J. 2013 Nov 12;11(1):24. doi: 10.1186/1477-9560-11-24.

Thrombin generation as marker to estimate thrombosis risk in patients with abnormal test results in lupus anticoagulant routine diagnostics.

Thrombosis journal

Klas Boeer, Leonid Cuznetov, Wolfgang Loesche

Affiliations

  1. Institut für Klinische Chemie und Laboratoriumsdiagnostik, Jena University Hospital - Friedrich Schiller University Jena, Erlanger Allee 101, 07747 Jena, Germany. [email protected].

PMID: 24219775 PMCID: PMC3842625 DOI: 10.1186/1477-9560-11-24

Abstract

BACKGROUND: Lupus anticoagulant (LA) is known to inhibit thrombin generation although patients have an increased risk to develop thrombosis. We tried to determine whether thrombin generation is altered in plasma samples of patients with abnormal test results in LA routine diagnostics and whether its measurement may improve the risk assessment of thrombosis.

METHODS: Samples from 63 patients (39 with abnormal test results; 24 controls) were included in the study. Measurement of diluted Russel's viper venom time (dRVVT) was part of the initial guideline conform diagnostic procedure for detection of LA. In addition, measurement of anticardiolipin-IgM, -IgG and β2-glycoprotein-I-IgM, -IgG were performed. Thrombin generation was measured using two different phospholipid concentrations in the starting reagent.

RESULTS: Analyzing all samples by logistic regression, thrombin generation after induction with high phospholipid concentrations was the best predictor of thrombosis. After preselection of samples with alterations in dRVVT, specificity of selected thrombin generation derived parameters for the detection of previous thrombosis increased in this subgroup.

CONCLUSIONS: In patients with phospholipid-dependent prolongation of dRVVT, thrombin generation is variably inhibited and the degree of inhibition corresponds to the occurrence of previous thrombosis. Measuring thrombin generation in patients with phospholipid-dependent dRVVT prolongation may improve risk assessment of thrombosis.

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