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Ahmad Salarian A, Jalali A, Zare Mirakabadi A, et al. Cytotoxic Effects of two Iranian Scorpions Odontobuthusdoriae and Bothutus saulcyi on Five Human Cultured Cell lines and Fractions of Toxic Venom. Iran J Pharm Res. 2012;11(1):357-67
Ahmad Salarian, A., Jalali, A., Zare Mirakabadi, A., Vatanpour, H., & H Shirazi, F. (2012). Cytotoxic Effects of two Iranian Scorpions Odontobuthusdoriae and Bothutus saulcyi on Five Human Cultured Cell lines and Fractions of Toxic Venom. Iranian journal of pharmaceutical research : IJPR, 11(1), 357-67.
Ahmad Salarian, Amir, et al. "Cytotoxic Effects of two Iranian Scorpions Odontobuthusdoriae and Bothutus saulcyi on Five Human Cultured Cell lines and Fractions of Toxic Venom." Iranian journal of pharmaceutical research : IJPR vol. 11,1 (2012): 357-67.
Ahmad Salarian A, Jalali A, Zare Mirakabadi A, Vatanpour H, H Shirazi F. Cytotoxic Effects of two Iranian Scorpions Odontobuthusdoriae and Bothutus saulcyi on Five Human Cultured Cell lines and Fractions of Toxic Venom. Iran J Pharm Res. 2012;11(1):357-67. PMID: 24250459; PMCID: PMC3813092.
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Iran J Pharm Res. 2012;11(1):357-67.

Cytotoxic Effects of two Iranian Scorpions Odontobuthusdoriae and Bothutus saulcyi on Five Human Cultured Cell lines and Fractions of Toxic Venom.

Iranian journal of pharmaceutical research : IJPR

Amir Ahmad Salarian, Amir Jalali, Abbas Zare Mirakabadi, Hossein Vatanpour, Farshad H Shirazi

Affiliations

  1. Department of Toxicology, School of Pharmacy, Shahid Beheshti University of Medical Science, Tehran, Iran. ; Student Research committee, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

PMID: 24250459 PMCID: PMC3813092

Abstract

Scorpion venom toxicity is of major concern due to its influence on human activities and public health. We investigated the in-vitro process of cell death caused by two Iranian scorpions Odontobuthus doriae and Bothutus salceyi venom on human cell lines. The aim of this study was to provide further information about triggering cell death and suggestion of methods for the elimination of unwanted cells such as tumor cells. The cytotoxicity and apoptosis induced by effect of scorpion venoms on five established eukaryotic cell lines are analyzed on different human cell lines. All cultured cell lines were incubated with varying doses of scorpion venom for 24 h at 37°C. Control culture was treated with an equal amount of SFM. The percentage of cell survival was measured using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium (MTT) colorimetric assay. Our data demonstrated that Bothutus saulcyi, does not show cytotoxic effect on any of the used cell lines. Odontobuthus doriae, however, has resulted a dose dependent cytotoxic effect with maximum at 1 ug/mL on 1321N1 glioma like cell line. Then the cytotoxic venom of O. doriae was fractionated using Sephadex G50 gel chromatography. The toxic fractions on mouse used to Cytotoxicity assay on 1321 N1 cell line and data demonstrated that, the fraction F3 showed a dose dependent Cytotoxicity assay. Further studies to explode the mode of action of these venoms are recommended and purification of the toxic fraction should be done.

Keywords: 1321N1; Bothutus saulcyi; Odontobuthus doriae; Scorpion

References

  1. Am J Physiol Cell Physiol. 2005 Jun;288(6):C1451-60 - PubMed
  2. J Neurosci. 1999 Jul 15;19(14):5942-54 - PubMed
  3. FEBS Lett. 2005 Aug 1;579(19):4181-6 - PubMed
  4. J Neurooncol. 2005 May;73(1):1-7 - PubMed
  5. J Neurosci. 2003 Jul 2;23(13):5572-82 - PubMed
  6. Glia. 2006 Aug 15;54(3):223-33 - PubMed
  7. Toxicon. 2007 Feb;49(2):124-41 - PubMed
  8. Prog Biophys Mol Biol. 1993;59(1):1-21 - PubMed
  9. FASEB J. 2006 Sep;20(11):1892-4 - PubMed
  10. J Membr Biol. 2000 Jul 1;176(1):31-40 - PubMed
  11. Pflugers Arch. 2003 May;446(2):248-55 - PubMed
  12. Cell Signal. 1996 Jun;8(4):279-84 - PubMed
  13. Am J Physiol. 1993 Feb;264(2 Pt 1):C361-9 - PubMed
  14. Toxicon. 2007 Dec 15;50(8):1019-26 - PubMed
  15. Neuroreport. 2002 Mar 25;13(4):403-7 - PubMed
  16. Iran J Pharm Res. 2012 Summer;11(3):905-11 - PubMed
  17. Toxicon. 2003 Sep;42(3):229-37 - PubMed
  18. Acta Neuropathol. 2005 Apr;109(4):418-26 - PubMed
  19. Eur J Pharmacol. 2002 Nov 1;454(1):25-30 - PubMed
  20. Prostate. 2001 Mar 1;46(4):262-74 - PubMed
  21. FEBS Lett. 2006 Nov 13;580(26):6254-8 - PubMed
  22. J Neurol Neurosurg Psychiatry. 2002 Feb;72(2):262-5 - PubMed
  23. Neuron Glia Biol. 2006 Feb;2(1):39-49 - PubMed
  24. Glia. 2004 Apr 1;46(1):63-73 - PubMed
  25. Toxicon. 2002 Jan;40(1):23-31 - PubMed
  26. Am J Physiol Cell Physiol. 2004 Jun;286(6):C1399-409 - PubMed
  27. Toxicon. 2003 Jun;41(8):971-7 - PubMed
  28. Mol Neurobiol. 2004 Feb;29(1):61-71 - PubMed
  29. Neurosurgery. 2005 Feb;56(2):375-81; discussion 375-81 - PubMed
  30. Toxicon. 1994 Sep;32(9):1015-8 - PubMed
  31. J Membr Biol. 2005 Jun;205(3):159-73 - PubMed
  32. Eur J Biochem. 1999 Sep;264(2):287-300 - PubMed

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