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Balkow C, Wildner GF. Aspartate aminotransferases ofPanicum miliaceum L. andPanicum antidotale retz. : Inactivation and reconstitution. Planta. 1982;154(5):477-84doi: 10.1007/BF01267817.
Balkow, C., & Wildner, G. F. (1982). Aspartate aminotransferases ofPanicum miliaceum L. andPanicum antidotale retz. : Inactivation and reconstitution. Planta, 154(5), 477-84. https://doi.org/10.1007/BF01267817
Balkow, C, and Wildner, G F. "Aspartate aminotransferases ofPanicum miliaceum L. andPanicum antidotale retz. : Inactivation and reconstitution." Planta vol. 154,5 (1982): 477-84. doi: https://doi.org/10.1007/BF01267817
Balkow C, Wildner GF. Aspartate aminotransferases ofPanicum miliaceum L. andPanicum antidotale retz. : Inactivation and reconstitution. Planta. 1982 Sep;154(5):477-84. doi: 10.1007/BF01267817. PMID: 24276278.
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Planta. 1982 Sep;154(5):477-84. doi: 10.1007/BF01267817.

Aspartate aminotransferases ofPanicum miliaceum L. andPanicum antidotale retz. : Inactivation and reconstitution.

Planta

C Balkow, G F Wildner

Affiliations

  1. Abteilung Biologie der Ruhr-Universität, Lehrstuhl für Biochemie der Pflanzen, D-4630, Bochum 1, Federal Republic of Germany.

PMID: 24276278 DOI: 10.1007/BF01267817

Abstract

L-Aspartate: 2-oxoglutarate transaminase was isolated and partially purified from leaves ofPanicum miliaceum (C4, NAD-malic enzyme type) and ofPanicum antidotale (C4, NADP-malic enzyme type). In each preparation two isoenzymes with different kinetic properties could be characterized. The enzyme activity was irreversibly inhibited by 2-aminooxyacetic acid and by 2-amino-4-methoxy-3-butenoic acid. The first inhibitor reacted with pyridoxal 5-phosphate, and its inhibition could be reversed by the exchange of the modified coenzyme. The second inhibitor binds not only to the coenzyme pyridoxal 5-phosphate, but also to the apoprotein. The results of the dissociation and reconstitution experiments were in agreement with the kinetic data, showing that the mode of inactivation was different for 2-aminooxyacetic acid and 2-amino-4-methoxy-3-butenoic acid.

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