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Sabour MP, Ramsey U, Nagai J. Decreased frequency of the rat growth hormone transgene in mouse populations with or without selection for increased adult body weight. Theor Appl Genet. 1991;81(3):327-32doi: 10.1007/BF00228672.
Sabour, M. P., Ramsey, U., & Nagai, J. (1991). Decreased frequency of the rat growth hormone transgene in mouse populations with or without selection for increased adult body weight. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 81(3), 327-32. https://doi.org/10.1007/BF00228672
Sabour, M P, et al. "Decreased frequency of the rat growth hormone transgene in mouse populations with or without selection for increased adult body weight." TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik vol. 81,3 (1991): 327-32. doi: https://doi.org/10.1007/BF00228672
Sabour MP, Ramsey U, Nagai J. Decreased frequency of the rat growth hormone transgene in mouse populations with or without selection for increased adult body weight. Theor Appl Genet. 1991 Mar;81(3):327-32. doi: 10.1007/BF00228672. PMID: 24221261.
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Theor Appl Genet. 1991 Mar;81(3):327-32. doi: 10.1007/BF00228672.

Decreased frequency of the rat growth hormone transgene in mouse populations with or without selection for increased adult body weight.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

M P Sabour, U Ramsey, J Nagai

Affiliations

  1. Animal Research Centre, Agriculture Canada, K1A OC6, Ottawa, Ontario, Canada.

PMID: 24221261 DOI: 10.1007/BF00228672

Abstract

Frequencies of mice with the rat growth hormone (rGH) transgene were examined in lines derived from two genetic bases (P/W and P/C). The genetic bases were developed from males (P) with the rGH transgene, mated with non-transgenic females of different origin: a line previously selected for large body size (W) and a corresponding unselected control line (C). They were maintained for six generations under random mating with or without selection for increased 42-day body weight. The frequencies of P/W and P/C males with the rGH transgene wer 0.075 and 0.300, respectively at generation 0 of the genetic bases. They were significantly (P<0.05) lower than the expected frequency (about 0.5). At generation 6, the frequencies had decreased further both in selected and unselected lines (ranging from 0.025 to 0.125). Decreased frequencies of mice with the transgene were confirmed in a separate experiment testing segregation of the transgene. The reasons for these decreases are not clear. The results suggest that transgenes need to be monitored when transgenic animals are mated with animals of different origin.

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