Display options
Cite
Van't Hof J, Lamm SS, Bjerknes CA. Detection of replication initiation by a replicon family in DNA of synchronized pea (Pisum sativum) root cells using benzoylated naphthoylated DEAE-cellulose chromatography. Plant Mol Biol. 1987;9(2):77-86doi: 10.1007/BF00015640.
Van't Hof, J., Lamm, S. S., & Bjerknes, C. A. (1987). Detection of replication initiation by a replicon family in DNA of synchronized pea (Pisum sativum) root cells using benzoylated naphthoylated DEAE-cellulose chromatography. Plant molecular biology, 9(2), 77-86. https://doi.org/10.1007/BF00015640
Van't Hof, J, et al. "Detection of replication initiation by a replicon family in DNA of synchronized pea (Pisum sativum) root cells using benzoylated naphthoylated DEAE-cellulose chromatography." Plant molecular biology vol. 9,2 (1987): 77-86. doi: https://doi.org/10.1007/BF00015640
Van't Hof J, Lamm SS, Bjerknes CA. Detection of replication initiation by a replicon family in DNA of synchronized pea (Pisum sativum) root cells using benzoylated naphthoylated DEAE-cellulose chromatography. Plant Mol Biol. 1987 Mar;9(2):77-86. doi: 10.1007/BF00015640. PMID: 24276897.
Copy Download .nbib Format: NLM
Share it on

Plant Mol Biol. 1987 Mar;9(2):77-86. doi: 10.1007/BF00015640.

Detection of replication initiation by a replicon family in DNA of synchronized pea (Pisum sativum) root cells using benzoylated naphthoylated DEAE-cellulose chromatography.

Plant molecular biology

J Van't Hof, S S Lamm, C A Bjerknes

Affiliations

  1. Biology Department, Brookhaven National Laboratory, 11973, Upton, New York, USA.

PMID: 24276897 DOI: 10.1007/BF00015640

Abstract

Fractionated replicating DNA from pea was obtained from both synchronized cells just starting replication and from carbohydrate-starved cells ending replication. Benzoylated naphthoylated DEAE-cellulose chromatography of pulse-labeled DNA digested with EcoR I gave evidence that a family of replicons initiated replication 45 to 60 min after synchronized cells were released from the G1/S phase boundary. DNA from cells labeled in late S phase, on the other hand, showed no signs of additional replication initiations before entering G2 phase. Results with DNA from both early and late S phase cells comply with a model based on the premise that with short pulses of [(3)H]-thymidine the isotope is localized at replication forks and that longer pulses label both replication forks and recently replicated segments of double-stranded DNA. The model applies only to DNA subjected to fragmentation before chromatography.The results also suggest that benzoylated naphthoylated DEAE-cellulose chromatography is a useful means to isolate origins and replication forks from synchronized plant cells.

References

  1. Exp Cell Res. 1981 Dec;136(2):461-5 - PubMed
  2. Mol Cell Biol. 1985 Jan;5(1):85-92 - PubMed
  3. Exp Cell Res. 1980 Sep;129(1):231-7 - PubMed
  4. Mol Cell Biol. 1983 Feb;3(2):172-81 - PubMed
  5. J Mol Biol. 1978 Apr 15;120(3):401-22 - PubMed
  6. Biochemistry. 1978 Dec 26;17(26):5781-90 - PubMed
  7. J Cell Biol. 1973 Aug;58(2):410-8 - PubMed
  8. Exp Cell Res. 1984 Feb;150(2):379-89 - PubMed
  9. Genetics. 1981 May;98(1):143-56 - PubMed
  10. J Mol Biol. 1978 Mar 15;119(4):487-506 - PubMed
  11. J Mol Biol. 1973 Jul 5;77(3):391-404 - PubMed
  12. Anal Biochem. 1975 Feb;63(2):442-51 - PubMed
  13. DNA. 1985 Apr;4(2):157-64 - PubMed
  14. J Mol Biol. 1969 Mar 28;40(3):467-90 - PubMed
  15. Biochim Biophys Acta. 1971 Jan 1;228(1):117-26 - PubMed
  16. J Cell Biol. 1970 Nov 1;47(2):536-9 - PubMed
  17. J Cell Biol. 1975 Jan;64(1):89-97 - PubMed
  18. J Virol. 1977 Feb;21(2):694-712 - PubMed
  19. J Mol Biol. 1967 Jun 28;26(3):537-40 - PubMed
  20. Cell. 1982 Apr;28(4):767-79 - PubMed
  21. Biochim Biophys Acta. 1981 Apr 27;653(2):185-92 - PubMed

Publication Types