Display options
Cite
Cohen JL, Hisayasu GH, Strum SB. High pressure liquid chromatographic analysis of metoclopramide in serum. Pharm Res. 1984;1(1):43-5doi: 10.1023/A:1016334827197.
Cohen, J. L., Hisayasu, G. H., & Strum, S. B. (1984). High pressure liquid chromatographic analysis of metoclopramide in serum. Pharmaceutical research, 1(1), 43-5. https://doi.org/10.1023/A:1016334827197
Cohen, J L, et al. "High pressure liquid chromatographic analysis of metoclopramide in serum." Pharmaceutical research vol. 1,1 (1984): 43-5. doi: https://doi.org/10.1023/A:1016334827197
Cohen JL, Hisayasu GH, Strum SB. High pressure liquid chromatographic analysis of metoclopramide in serum. Pharm Res. 1984 Jan;1(1):43-5. doi: 10.1023/A:1016334827197. PMID: 24277185.
Copy Download .nbib Format: NLM
Share it on

Pharm Res. 1984 Jan;1(1):43-5. doi: 10.1023/A:1016334827197.

High pressure liquid chromatographic analysis of metoclopramide in serum.

Pharmaceutical research

J L Cohen, G H Hisayasu, S B Strum

Affiliations

  1. Clinical Pharmacokinetic Laboratory, School of Pharmacy University of Southern California, Los Angeles, California.

PMID: 24277185 DOI: 10.1023/A:1016334827197

Abstract

Metoclopramide has recently been approved at dose levels of 1 to 2 mg/kg for the treatment of nausea and vomiting resulting from cancer chemotherapeutic agents. A rapid, sensitive reverse phase HPLC quantitative procedure for metoclopramide in serum is described. The method involves a single-step extraction of metoclopramide and disopyramide (internal standard) from alkalinized serum into benzene and utilizes a reverse-phase C-8 system with a mobile phase of 11:22:66, methanol: acetonitrile: pH 3.7 acetate buffer, and UV detection at 268 nm. The method is highly reproducible and has a limit of sensitivity of 2.5 ng/ml from a 2.0 ml serum sample. The method has been successfully applied to clinical pharmacokinetic studies involving administration of IV oral metoclopramide to cancer patients receiving highly emetogenic cis-diamminedichloroplatinum.

References

  1. JAMA. 1982 May 21;247(19):2683-6 - PubMed
  2. N Engl J Med. 1981 Oct 15;305(16):905-9 - PubMed
  3. Br J Clin Pharmacol. 1978 Nov;6(5):401-7 - PubMed
  4. Br J Clin Pharmacol. 1979 Nov;8(5):469-74 - PubMed
  5. Drugs. 1976;12(2):81-131 - PubMed
  6. Eur J Clin Pharmacol. 1981;19(6):437-41 - PubMed
  7. Eur J Clin Pharmacol. 1981;20(6):465-71 - PubMed
  8. Arzneimittelforschung. 1981;31(6):1041-5 - PubMed
  9. J Pharm Pharmacol. 1976 Jan;28(1):32-9 - PubMed
  10. J Pharm Sci. 1978 Aug;67(8):1073-7 - PubMed
  11. J Pharm Sci. 1977 Nov;66(11):1615-8 - PubMed

Publication Types