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Vandenbosch KA, Newcomb EH. The occurrence of leghemoglobin protein in the uninfected interstitial cells of soybean root nodules. Planta. 1988;175(4):442-51doi: 10.1007/BF00393063.
Vandenbosch, K. A., & Newcomb, E. H. (1988). The occurrence of leghemoglobin protein in the uninfected interstitial cells of soybean root nodules. Planta, 175(4), 442-51. https://doi.org/10.1007/BF00393063
Vandenbosch, K A, and Newcomb, E H. "The occurrence of leghemoglobin protein in the uninfected interstitial cells of soybean root nodules." Planta vol. 175,4 (1988): 442-51. doi: https://doi.org/10.1007/BF00393063
Vandenbosch KA, Newcomb EH. The occurrence of leghemoglobin protein in the uninfected interstitial cells of soybean root nodules. Planta. 1988 Oct;175(4):442-51. doi: 10.1007/BF00393063. PMID: 24221924.
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Planta. 1988 Oct;175(4):442-51. doi: 10.1007/BF00393063.

The occurrence of leghemoglobin protein in the uninfected interstitial cells of soybean root nodules.

Planta

K A Vandenbosch, E H Newcomb

Affiliations

  1. Department of Botany, University of Wisconsin, 53706, Madison, WI, USA.

PMID: 24221924 DOI: 10.1007/BF00393063

Abstract

The distribution of leghemoglobin (Lb) in resin-embedded root nodules of soybean (Glycine max (L.) Merr.) was investigated using immunogold labeling. Using anti-Lb immunoglobulin G and protein A-gold, Lb or its apoprotein was detected both in cells infected by Bradyrhizobium japonicum and in uninfected interstitial cells. Leghemoglobin was present in the cytoplasm, exclusive of the organelles, and in the nuclei of both cell types. In a comparison of the density of labeling in adjacent pairs of infected and uninfected cells, Lb was found to be about four times more concentrated in infected cells. This is the first report of Lb in uninfected cells of any legume nodule; it raises the possibility that this important nodule-specific protein may participate in mediating oxygen flow to host plant organelles throughout the infected region of the nodule.

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