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Mitchell JG, Weller R, Beconi M, et al. A practical optical trap for manipulating and isolating bacteria from complex microbial communities. Microb Ecol. 1993;25(2):113-9doi: 10.1007/BF00177189.
Mitchell, J. G., Weller, R., Beconi, M., Sell, J., & Holland, J. (1993). A practical optical trap for manipulating and isolating bacteria from complex microbial communities. Microbial ecology, 25(2), 113-9. https://doi.org/10.1007/BF00177189
Mitchell, J G, et al. "A practical optical trap for manipulating and isolating bacteria from complex microbial communities." Microbial ecology vol. 25,2 (1993): 113-9. doi: https://doi.org/10.1007/BF00177189
Mitchell JG, Weller R, Beconi M, Sell J, Holland J. A practical optical trap for manipulating and isolating bacteria from complex microbial communities. Microb Ecol. 1993 Mar;25(2):113-9. doi: 10.1007/BF00177189. PMID: 24189809.
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Microb Ecol. 1993 Mar;25(2):113-9. doi: 10.1007/BF00177189.

A practical optical trap for manipulating and isolating bacteria from complex microbial communities.

Microbial ecology

J G Mitchell, R Weller, M Beconi, J Sell, J Holland

Affiliations

  1. School of Biological Sciences, Flinders University, GPO 2100, 5001, Adelaide, Australia.

PMID: 24189809 DOI: 10.1007/BF00177189

Abstract

A wide variety of naturally occurring bacteria cannot be isolated by classical microbiological methods, hindering study of microbial communities. To partially remove this limitation, a method using optical trapping was developed. It allows for one-step isolation of bacteria from a complex community to a pure culture. The method transfers a single bacterium to sterile culture medium using a non-destructive laser beam. The ease with which a previously unculturable cell was cultured by trapping suggests that competition may limit growth in some natural communities of bacteria.

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