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Zirrolli JA, Wheelan P, Murphy RC. Analysis of long-chain fatty acyl coenzyme a thioesters by negative ion fast-atom bombardment mass spectrometry and tandem mass spectrometry. J Am Soc Mass Spectrom. 1994;5(5):416-24doi: 10.1016/1044-0305(94)85057-7.
Zirrolli, J. A., Wheelan, P., & Murphy, R. C. (1994). Analysis of long-chain fatty acyl coenzyme a thioesters by negative ion fast-atom bombardment mass spectrometry and tandem mass spectrometry. Journal of the American Society for Mass Spectrometry, 5(5), 416-24. https://doi.org/10.1016/1044-0305(94)85057-7
Zirrolli, J A, et al. "Analysis of long-chain fatty acyl coenzyme a thioesters by negative ion fast-atom bombardment mass spectrometry and tandem mass spectrometry." Journal of the American Society for Mass Spectrometry vol. 5,5 (1994): 416-24. doi: https://doi.org/10.1016/1044-0305(94)85057-7
Zirrolli JA, Wheelan P, Murphy RC. Analysis of long-chain fatty acyl coenzyme a thioesters by negative ion fast-atom bombardment mass spectrometry and tandem mass spectrometry. J Am Soc Mass Spectrom. 1994 May;5(5):416-24. doi: 10.1016/1044-0305(94)85057-7. PMID: 24222596.
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J Am Soc Mass Spectrom. 1994 May;5(5):416-24. doi: 10.1016/1044-0305(94)85057-7.

Analysis of long-chain fatty acyl coenzyme a thioesters by negative ion fast-atom bombardment mass spectrometry and tandem mass spectrometry.

Journal of the American Society for Mass Spectrometry

J A Zirrolli, P Wheelan, R C Murphy

Affiliations

  1. Department of Pediatrics, National Jewish Center for Immunology and Respiratory Medicine, 1400 Jackson Street, 80206, Denver, CO, USA.

PMID: 24222596 DOI: 10.1016/1044-0305(94)85057-7

Abstract

Long-chain acyl Coenzyme A (CoA) is essentially composed of three major chemical groups, fatty acyl-, phosphopantetheino-, and 3', 5',-adenosine diphospho-moieties. The negative ion fast-atom bombardment mass spectrometry spectra of long-chain acyl CoA thioesters were characterized by the formation of abundant [M - H](-) and two distinct classes of fragment ions, one class which retained the acyl group and another class which is related to CoA that contains the phosphopantethene and adenine. The ions which retained the acyl group in the spectrum of palmitoyl CoA appeared at m/z 675, 657, 595, and 577 and were found to decompose by loss of alkylketene observed at m/z 357 and 339. Those ions which retained the adenine group were observed at m/z 426 and 408. In contrast to these ions observed following fast-atom bombardment ionization, tandem mass spectrometry of the [M - H](-), from palmitoyl CoA (m/z 1004), yielded the adenine-containing ions as major products and the acyl-containing ions were of low abundance or not detected. These results suggested that the formation of many characteristic ions observed in direct FAB analysis occurred during the desorption process. The unique relationship between ions which involved the transition from acyl-containing ions to only CoA-containing ions by the loss of alkylketene allowed the development of tandem mass spectrometry protocols for the analysis of acyl CoA mixtures. Precursor scans of either m/z 357 or 339 yielded the identification of each species in a complex mixture. Identification of specific species was obtained with a neutral loss scan of the mass for a specific alkylketene.

References

  1. Biochim Biophys Acta. 1992 Mar 4;1124(2):101-11 - PubMed
  2. J Chromatogr. 1991 Jan 2;562(1-2):47-58 - PubMed
  3. Annu Rev Biochem. 1977;46:263-98 - PubMed
  4. J Chromatogr. 1990 Jun 22;509(2):325-32 - PubMed
  5. Biochemistry. 1991 Jan 15;30(2):388-94 - PubMed
  6. Biochem Biophys Res Commun. 1988 Aug 15;154(3):1248-52 - PubMed
  7. J Chromatogr. 1990 May 18;527(2):289-301 - PubMed
  8. Anal Chem. 1991 May 15;63(10):1032-8 - PubMed
  9. J Lipid Res. 1993 May;34(5):691-702 - PubMed
  10. Drug Metab Dispos. 1986 Jul-Aug;14(4):465-70 - PubMed
  11. Drug Metab Dispos. 1992 Mar-Apr;20(2):234-40 - PubMed
  12. J Biol Chem. 1990 Sep 25;265(27):16288-95 - PubMed
  13. J Am Soc Mass Spectrom. 1991 Jan;2(1):45-54 - PubMed
  14. Anal Biochem. 1990 Feb 1;184(2):311-6 - PubMed
  15. J Lipid Res. 1993 Jul;34(7):1229-35 - PubMed
  16. Lipids. 1986 Sep;21(9):580-8 - PubMed
  17. Lipids. 1991 Dec;26(12):1112-6 - PubMed

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