Display options
Cite
Karnaukhova EN, Reshetova OS, Semenov SY, et al. (2)H- and(13)C-labeled amino acids generated by obligate methylotrophs Biosynthesis and MS monitoring. Amino Acids. 1994;6(2):165-76doi: 10.1007/BF00805844.
Karnaukhova, E. N., Reshetova, O. S., Semenov, S. Y., Skladnev, D. A., & Tsygankov, Y. D. (1994). (2)H- and(13)C-labeled amino acids generated by obligate methylotrophs Biosynthesis and MS monitoring. Amino acids, 6(2), 165-76. https://doi.org/10.1007/BF00805844
Karnaukhova, E N, et al. "(2)H- and(13)C-labeled amino acids generated by obligate methylotrophs Biosynthesis and MS monitoring." Amino acids vol. 6,2 (1994): 165-76. doi: https://doi.org/10.1007/BF00805844
Karnaukhova EN, Reshetova OS, Semenov SY, Skladnev DA, Tsygankov YD. (2)H- and(13)C-labeled amino acids generated by obligate methylotrophs Biosynthesis and MS monitoring. Amino Acids. 1994 Jun;6(2):165-76. doi: 10.1007/BF00805844. PMID: 24190786.
Copy Download .nbib Format: NLM
Share it on

Amino Acids. 1994 Jun;6(2):165-76. doi: 10.1007/BF00805844.

(2)H- and(13)C-labeled amino acids generated by obligate methylotrophs Biosynthesis and MS monitoring.

Amino acids

E N Karnaukhova, O S Reshetova, S Y Semenov, D A Skladnev, Y D Tsygankov

Affiliations

  1. Department of Biotechnology, Lomonosov Institute of Fine Chemical Technology, Pr. Vernadskogo 86, 117571, Moscow, Russia.

PMID: 24190786 DOI: 10.1007/BF00805844

Abstract

Biosynthetic preparation of(2)H- and(13)C- labeled amino acids was studied using a leucine-producing mutant of the obligate methylotroph,Methylobacillus flagellatum. The strain was cultivated in various media containing(13)C- or(2)H-analogs of methanol. The total protein from each experiment was subjected to acid hydrolysis and converted into a mixture of dansyl amino acid methyl esters. The samples of excreted leucine were converted into methyl esters of dansyl and benzyloxycarbonyl derivatives. Electron impact mass spectrometry was performed to detect stable isotope enrichment of the amino acids. According to the mass spectrometric analysis it is feasible to use methylotrophic microorganisms for the preparation of(2)H- and(13)C- analogs of amino acids by labeled methanol bioconversion; the excreted amino acids can be convenient for express analysis as an indicator of isotopic enrichment of the total protein. The data obtained testified to a high efficiency of dansyl derivatization for mass spectrometric analysis of complex amino acid mixtures.

References

  1. Biomed Mass Spectrom. 1985 Aug;12(8):432-6 - PubMed
  2. Anal Biochem. 1989 Sep;181(2):271-5 - PubMed
  3. J Biol Chem. 1990 Dec 25;265(36):22059-62 - PubMed
  4. Methods Enzymol. 1989;177:23-43 - PubMed
  5. Biochim Biophys Acta. 1970 Jan 20;200(1):26-33 - PubMed
  6. Science. 1968 Aug 23;161(3843):795-6 - PubMed
  7. Anal Biochem. 1990 Nov 15;191(1):9-15 - PubMed
  8. Q Rev Biophys. 1990 May;23(2):133-74 - PubMed
  9. Anal Biochem. 1988 Nov 1;174(2):374-80 - PubMed
  10. Q Rev Biophys. 1990 Feb;23(1):1-38 - PubMed
  11. Q Rev Biophys. 1990 May;23(2):97-131 - PubMed
  12. Q Rev Biophys. 1987 Feb;19(1-2):51-82 - PubMed
  13. Anal Biochem. 1990 Feb 15;185(1):1-9 - PubMed

Publication Types