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Khazaei M, Moshayedi MA, Jervekani MT, et al. The effect of L-arginine and L-NAME on myocardial capillary density in normal rats. Adv Biomed Res. 2013;2:67doi: 10.4103/2277-9175.115819.
Khazaei, M., Moshayedi, M. A., Jervekani, M. T., Aghili, S., Montazer, S., Dastjerdi, R. M., Hashemzehi, F., & Jazi, H. H. (2013). The effect of L-arginine and L-NAME on myocardial capillary density in normal rats. Advanced biomedical research, 267. https://doi.org/10.4103/2277-9175.115819
Khazaei, Majid, et al. "The effect of L-arginine and L-NAME on myocardial capillary density in normal rats." Advanced biomedical research vol. 2 (2013): 67. doi: https://doi.org/10.4103/2277-9175.115819
Khazaei M, Moshayedi MA, Jervekani MT, Aghili S, Montazer S, Dastjerdi RM, Hashemzehi F, Jazi HH. The effect of L-arginine and L-NAME on myocardial capillary density in normal rats. Adv Biomed Res. 2013 Jul 30;2:67. doi: 10.4103/2277-9175.115819. eCollection 2013. PMID: 24223382; PMCID: PMC3814566.
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Adv Biomed Res. 2013 Jul 30;2:67. doi: 10.4103/2277-9175.115819. eCollection 2013.

The effect of L-arginine and L-NAME on myocardial capillary density in normal rats.

Advanced biomedical research

Majid Khazaei, Muhammad Amin Moshayedi, Massoud Teimouri Jervekani, Shahrzad Aghili, Saeed Montazer, Roshanak Mehdipour Dastjerdi, Fazlolah Hashemzehi, Hourossadat Hashemi Jazi

Affiliations

  1. Department of Physiology, Isfahan University of Medical Sciences, Isfahan, Iran.

PMID: 24223382 PMCID: PMC3814566 DOI: 10.4103/2277-9175.115819

Abstract

BACKGROUND: This study evaluated the effect of L-arginine (Nitric Oxide (NO) precursor) and L-NG-Nitroarginine Methyl Ester (L-NAME) (NO synthase inhibitor) on myocardial capillary density in normal rats.

MATERIALS AND METHODS: EIGHTEEN MALE RATS WERE DIVIDED INTO THREE GROUPS: Group 1: Received L-NAME (10 mg/kg/day; ip), Group 2: Received L-arginine (50 mg/kg/day; ip), and Group 3 (control) received normal saline. After 3 weeks, blood samples were taken and myocardial capillary density was evaluated using immunohistochemistry method.

RESULTS: Serum NO concentration in control group was 6.45 ± 0.44 μmol/lit. Treatment of animals with L-arginine increased serum NO concentration (7.90 ± 0.75 vs. 6.45 ± 0.44 μmol/lit, respectively) and L-NAME decreased (4.86 ± 0.40 vs. 6.45 ± 0.44 μmol/lit, respectively) compare to control group. L-arginine significantly increased serum vascular endothelial growth factor (VEGF) concentration (353.01 ± 7.03 vs. 100.5 ± 6.61 pg/ml; P < 0.05), however, did not change myocardial capillary density.

CONCLUSION: Although L-arginine alters some serum angiogenic factors, either L-arginine or L-NAME could not improve myocardial capillary density in normal rats.

Keywords: Angiogenesis; L-NG-Nitroarginine Methyl Ester; L-arginine; myocardium

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