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Hem L, Khay S, Choi JH, et al. Determination of Trichlorfon Pesticide Residues in Milk via Gas Chromatography with μ-Electron Capture Detection and GC-MS. Toxicol Res. 2010;26(2):149-55doi: 10.5487/TR.2010.26.2.149.
Hem, L., Khay, S., Choi, J. H., Morgan, E. D., Abd El-Aty, A. M., & Shim, J. H. (2010). Determination of Trichlorfon Pesticide Residues in Milk via Gas Chromatography with μ-Electron Capture Detection and GC-MS. Toxicological research, 26(2), 149-55. https://doi.org/10.5487/TR.2010.26.2.149
Hem, Lina, et al. "Determination of Trichlorfon Pesticide Residues in Milk via Gas Chromatography with μ-Electron Capture Detection and GC-MS." Toxicological research vol. 26,2 (2010): 149-55. doi: https://doi.org/10.5487/TR.2010.26.2.149
Hem L, Khay S, Choi JH, Morgan ED, Abd El-Aty AM, Shim JH. Determination of Trichlorfon Pesticide Residues in Milk via Gas Chromatography with μ-Electron Capture Detection and GC-MS. Toxicol Res. 2010 Jun;26(2):149-55. doi: 10.5487/TR.2010.26.2.149. PMID: 24278518; PMCID: PMC3834475.
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Toxicol Res. 2010 Jun;26(2):149-55. doi: 10.5487/TR.2010.26.2.149.

Determination of Trichlorfon Pesticide Residues in Milk via Gas Chromatography with μ-Electron Capture Detection and GC-MS.

Toxicological research

Lina Hem, Sathya Khay, Jeong-Heui Choi, E D Morgan, A M Abd El-Aty, Jae-Han Shim

Affiliations

  1. Natural Products Chemistry Laboratory, Division of Applied Bioscience and Biotechnology, College of Agriculture and Life Science, Chonnam National University, Gwangju 500-757, Korea.

PMID: 24278518 PMCID: PMC3834475 DOI: 10.5487/TR.2010.26.2.149

Abstract

The pesticide trichlorfon is readily degraded under experimental conditions to dichlorvos. A method has therefore been developed by which residues of trichlorfon in milk are determined as dichlorvos, using gas chromatography with μ-electron capture detection. The identification of dichlorvos was confirmed by mass spectrometry. Milk was extracted with acetonitrile followed by centrifugation, freezing lipid filtration, and partitioning into dichloromethane. The residue after partitioning of dichloromethane was dissolved in ethyl acetate for gas chromatography. Recovery concentration was determined at 0.5, 1.0, and 2.0 of times the maximum permitted residue limits (MRLs) for trichlorfon in milk. The average recoveries (n = 6) ranged from 92.4 to 103.6%. The repeatability of the measurements was expressed as relative standard deviations (RSDs) ranging from 3.6%, to 6.7%. Limit of detection (LOD) and limit of quantification (LOQ) were 3.7 and 11.1 μg/l, respectively. The accuracy and precision (expressed as RSD) were estimated at concentrations from 25 to 250 μg/l. The intra- and inter-day accuracy (n = 6) ranged from 89.2%to 91% and 91.3% to 96.3%, respectively. The intra- and inter-day precisions were lower than 8%. The developed method was applied to determine trichlorfon in real samples collected from the seven major cities in the Republic of Korea. No residual trichlorfon was detected in any samples.

Keywords: Dichlorvos; Gas chromatography; Mass spectrometry; Milk; Trichlorfon

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