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Nolan CV, Shaikh ZA. Induction of metallothionein in rat tissues following subchronic exposure to mercury shown by radioimmunoassay. Biol Trace Elem Res. 1987;12(1):419-28doi: 10.1007/BF02796698.
Nolan, C. V., & Shaikh, Z. A. (1987). Induction of metallothionein in rat tissues following subchronic exposure to mercury shown by radioimmunoassay. Biological trace element research, 12(1), 419-28. https://doi.org/10.1007/BF02796698
Nolan, C V, and Shaikh, Z A. "Induction of metallothionein in rat tissues following subchronic exposure to mercury shown by radioimmunoassay." Biological trace element research vol. 12,1 (1987): 419-28. doi: https://doi.org/10.1007/BF02796698
Nolan CV, Shaikh ZA. Induction of metallothionein in rat tissues following subchronic exposure to mercury shown by radioimmunoassay. Biol Trace Elem Res. 1987 Apr;12(1):419-28. doi: 10.1007/BF02796698. PMID: 24254621.
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Biol Trace Elem Res. 1987 Apr;12(1):419-28. doi: 10.1007/BF02796698.

Induction of metallothionein in rat tissues following subchronic exposure to mercury shown by radioimmunoassay.

Biological trace element research

C V Nolan, Z A Shaikh

Affiliations

  1. Department of Pharmacology and Toxicology, University of Rhode Island, 02881-0809, Kingston, RI.

PMID: 24254621 DOI: 10.1007/BF02796698

Abstract

Although the analysis of metallothionein (MT) by radioimmunoassay (RIA) is not a common technique, its use is preferred over other methods since it offers the advantages of sensitivity and specificity. In this paper we present data on the basal levels of MT in rat tissues and physiological fluids of female Sprague-Dawley rats. The mean basal MT concentrations of the following organs and fluids were determined by RIA to be: liver (9.8 μg/g), kidney (68 μ/g), brain (0.8 μg/g), spleen (1.0 μg/g), heart (5.4 μg/g), plasma (11 ng/ml), and urine (200-300 μg/g creatinine). Following subcutaneous exposure to inorganic mercury (0.2 μmol/kg/d, 5 d a week for up to 4 wk), the metal accumulated primarily in the kidney. There was also a simultaneous accumulation of zinc in the liver and of zinc and copper in the kidney. Induction of MT did take place in liver, kidney, brain, and spleen. No increases in the MT contents of blood and urine were noted. The excess zinc and copper in the kidney of exposed animals were found to be associated predominantly with MT. No overt signs of mercury toxicity were noted in these animals and the incidence of proteinurea was nil. The data are discussed with reference to methods of MT determination in animal tissues and in relation to mercury metabolism and toxicity.

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