Display options
Cite
Ichihara K. The action of phosphatidate phosphatase on the fatty-acid composition of safflower triacylglycerol and spinach glycerolipids. Planta. 1991;183(3):353-8doi: 10.1007/BF00197733.
Ichihara, K. (1991). The action of phosphatidate phosphatase on the fatty-acid composition of safflower triacylglycerol and spinach glycerolipids. Planta, 183(3), 353-8. https://doi.org/10.1007/BF00197733
Ichihara, K. "The action of phosphatidate phosphatase on the fatty-acid composition of safflower triacylglycerol and spinach glycerolipids." Planta vol. 183,3 (1991): 353-8. doi: https://doi.org/10.1007/BF00197733
Ichihara K. The action of phosphatidate phosphatase on the fatty-acid composition of safflower triacylglycerol and spinach glycerolipids. Planta. 1991 Feb;183(3):353-8. doi: 10.1007/BF00197733. PMID: 24193745.
Copy Download .nbib Format: NLM
Share it on

Planta. 1991 Feb;183(3):353-8. doi: 10.1007/BF00197733.

The action of phosphatidate phosphatase on the fatty-acid composition of safflower triacylglycerol and spinach glycerolipids.

Planta

K Ichihara

Affiliations

  1. Department of Agricultural Chemistry, Kyoto Prefectural University, Shimogamo, 606, Kyoto, Japan.

PMID: 24193745 DOI: 10.1007/BF00197733

Abstract

The microsomal phosphatidate phosphatase (EC 3.1.3.4) in maturing seeds of safflower (Carthamus tinctorius L.) was specific and selective for unsaturated phosphatidates. The relative order of specificity for phosphatidate molecular species was 1,2-dilinoleoyl = 1,2-dioleoyl > 1-palmitoyl-2-oleoyl > 1,2-dilauroyl = 1,2-dimyristoyl > 1,2-dipalmitoyl. The order of selectivity was similar to that of the specificity. The broad selectivity for unsaturated phosphatidate species (1,2-di-unsaturated-acyl and 1-saturated-acyl-2-unsaturatedacyl) led us to conclude that the phosphatidate-phosphatase reaction does not, or only very little, affect the fatty-acid composition of the diacylglycerol product and in turn the fatty-acid composition of triacylglycerol in safflower oil. As compared with the safflower microsomal enzyme, the chloroplast phosphatidate phosphatase of spinach (Spinacia oleracea L.) leaves showed a broader specificity. This agreed with the selectivity profile indicated by labelling patterns of phosphatidate and diacylglycerol synthesized from [(14)C]acetate in spinach chloroplasts (S.E. Gardiner et al. 1984, Biochem. J. 224, 637-643).

References

  1. Biochem J. 1985 Sep 1;230(2):379-88 - PubMed
  2. FEBS Lett. 1979 Jun 1;102(1):147-50 - PubMed
  3. Plant Physiol. 1987 Jul;84(3):762-5 - PubMed
  4. Eur J Biochem. 1971 Jan;18(2):214-20 - PubMed
  5. Can J Biochem. 1981 Jul;59(7):500-10 - PubMed
  6. Arch Biochem Biophys. 1984 Aug 1;232(2):685-98 - PubMed
  7. Plant Physiol. 1980 Nov;66(5):1001-7 - PubMed
  8. Biochim Biophys Acta. 1990 Apr 17;1043(3):227-34 - PubMed
  9. Biochim Biophys Acta. 1977 Feb 23;486(2):359-68 - PubMed
  10. Biochim Biophys Acta. 1978 Sep 28;530(3):347-56 - PubMed
  11. Biochim Biophys Acta. 1983 Jul 12;752(2):198-208 - PubMed
  12. Biochim Biophys Acta. 1988 Jan 19;958(1):125-9 - PubMed
  13. Anal Biochem. 1985 Dec;151(2):479-86 - PubMed
  14. Biochem J. 1984 Dec 1;224(2):637-43 - PubMed
  15. Planta. 1974 Jun;118(2):159-69 - PubMed
  16. Eur J Biochem. 1987 Sep 1;167(2):339-47 - PubMed
  17. J Lipid Res. 1979 Jul;20(5):674-7 - PubMed
  18. J Biol Chem. 1951 Nov;193(1):265-75 - PubMed
  19. Plant Physiol. 1989 Jun;90(2):413-9 - PubMed

Publication Types