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Sabater M, Rubery PH. Auxin carriers in Cucurbita vesicles : I. Imposed perturbations of transmembrane pH and electrical potential gradients characterised by radioactive probes. Planta. 1987;171(4):501-6doi: 10.1007/BF00392298.
Sabater, M., & Rubery, P. H. (1987). Auxin carriers in Cucurbita vesicles : I. Imposed perturbations of transmembrane pH and electrical potential gradients characterised by radioactive probes. Planta, 171(4), 501-6. https://doi.org/10.1007/BF00392298
Sabater, M, and Rubery, P H. "Auxin carriers in Cucurbita vesicles : I. Imposed perturbations of transmembrane pH and electrical potential gradients characterised by radioactive probes." Planta vol. 171,4 (1987): 501-6. doi: https://doi.org/10.1007/BF00392298
Sabater M, Rubery PH. Auxin carriers in Cucurbita vesicles : I. Imposed perturbations of transmembrane pH and electrical potential gradients characterised by radioactive probes. Planta. 1987 Aug;171(4):501-6. doi: 10.1007/BF00392298. PMID: 24225712.
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Planta. 1987 Aug;171(4):501-6. doi: 10.1007/BF00392298.

Auxin carriers in Cucurbita vesicles : I. Imposed perturbations of transmembrane pH and electrical potential gradients characterised by radioactive probes.

Planta

M Sabater, P H Rubery

Affiliations

  1. Department of Biochemistry, University of Cambridge, CB2 1QW, Cambridge, UK.

PMID: 24225712 DOI: 10.1007/BF00392298

Abstract

The behaviour of radioactive probes of transmembrane pH gradients ([(14)C]butyric acid) and electrical potential gradients ((86)Rb(+) and S(14)CN(-)) has been characterised in membrane vesicles from Cucurbita pepo L. hypocotyls subjected to changing gradients and ionophore-mediated changes in the conductivity of K(+) and H(+) ions. The vesicles are sealed in the presence of 25 mM KCl. The K(+) ionophore valinomycin changes the distribution of (86)Rb(+) and of S(14)CN(-) in opposite directions, which depend on the external K(+) concentration. The accumulation of [(14)C]butyric acid by the vesicles and its association with the membranous phase are enhanced by increasing acidity, as expected for this lipophilic weak acid (pKa=4.8). Uptake of S(14)CN(-) increases and valinomycin-stimulated (86)Rb(+) uptake decreases when the external pH is lowered, indicating a strongly pH-dependent H(+) diffusion potential.

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