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Inaba K, Seno M, Morino H. Expression patterns of acidic and basic fibroblast growth factor in loach fish embryos. Fish Physiol Biochem. 1996;15(4):333-8doi: 10.1007/BF02112360.
Inaba, K., Seno, M., & Morino, H. (1996). Expression patterns of acidic and basic fibroblast growth factor in loach fish embryos. Fish physiology and biochemistry, 15(4), 333-8. https://doi.org/10.1007/BF02112360
Inaba, K, et al. "Expression patterns of acidic and basic fibroblast growth factor in loach fish embryos." Fish physiology and biochemistry vol. 15,4 (1996): 333-8. doi: https://doi.org/10.1007/BF02112360
Inaba K, Seno M, Morino H. Expression patterns of acidic and basic fibroblast growth factor in loach fish embryos. Fish Physiol Biochem. 1996 Aug;15(4):333-8. doi: 10.1007/BF02112360. PMID: 24194252.
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Fish Physiol Biochem. 1996 Aug;15(4):333-8. doi: 10.1007/BF02112360.

Expression patterns of acidic and basic fibroblast growth factor in loach fish embryos.

Fish physiology and biochemistry

K Inaba, M Seno, H Morino

Affiliations

  1. Biological Institute, Hyogo University of Teachers Education, Yashiro, Kato-gun, 673-14, Hyogo, Japan.

PMID: 24194252 DOI: 10.1007/BF02112360

Abstract

Heparin-binding fractions were taken from the heparin sepharose columns on which extracts of loach fish (Misgurnus anguillicaudatus) embryos from blastula, gastrula, 4-8 and 12-16 somites stages were applied. These heparin-binding fractions, except the fraction derived from 12-16 somite embryos, showed potent mitogenic activity on fibroblast-like cells derived from caudal fin blastema of goldfish. Western blot analysis of these heparin-binding fractions was carried out using monoclonal antibodies against human acidic and basic fibroblast growth factors (FGF-1 and-2). An immunoreactive FGF-1 band at 16 kD was detected in the heparin-binding fraction derived from embryos in each stage of blastula, gastrula and 4-8 somites. An immunoreactive FGF-2 band at 17 kDa was detected exclusively in the heparin-binding fraction derived from 4-8 somite embryos. In the heparin-binding fraction derived from 12-16 somite embryos neither immunoreactive FGF-1 nor-2 member band was detectable.

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