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Mezaki Y, Morii M, Hebiguchi T, et al. Differential increases in the expression of intermediate filament proteins and concomitant morphological changes of transdifferentiating rat hepatic stellate cells observed in vitro. Acta Histochem Cytochem. 2013;46(5):137-43doi: 10.1267/ahc.13007.
Mezaki, Y., Morii, M., Hebiguchi, T., Yoshikawa, K., Yamaguchi, N., Miura, M., Imai, K., Yoshino, H., & Senoo, H. (2013). Differential increases in the expression of intermediate filament proteins and concomitant morphological changes of transdifferentiating rat hepatic stellate cells observed in vitro. Acta histochemica et cytochemica, 46(5), 137-43. https://doi.org/10.1267/ahc.13007
Mezaki, Yoshihiro, et al. "Differential increases in the expression of intermediate filament proteins and concomitant morphological changes of transdifferentiating rat hepatic stellate cells observed in vitro." Acta histochemica et cytochemica vol. 46,5 (2013): 137-43. doi: https://doi.org/10.1267/ahc.13007
Mezaki Y, Morii M, Hebiguchi T, Yoshikawa K, Yamaguchi N, Miura M, Imai K, Yoshino H, Senoo H. Differential increases in the expression of intermediate filament proteins and concomitant morphological changes of transdifferentiating rat hepatic stellate cells observed in vitro. Acta Histochem Cytochem. 2013 Oct 30;46(5):137-43. doi: 10.1267/ahc.13007. Epub 2013 Oct 23. PMID: 24194627; PMCID: PMC3813821.
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Acta Histochem Cytochem. 2013 Oct 30;46(5):137-43. doi: 10.1267/ahc.13007. Epub 2013 Oct 23.

Differential increases in the expression of intermediate filament proteins and concomitant morphological changes of transdifferentiating rat hepatic stellate cells observed in vitro.

Acta histochemica et cytochemica

Yoshihiro Mezaki, Mayako Morii, Taku Hebiguchi, Kiwamu Yoshikawa, Noriko Yamaguchi, Mitsutaka Miura, Katsuyuki Imai, Hiroaki Yoshino, Haruki Senoo

Affiliations

  1. Department of Cell Biology and Morphology, Akita University Graduate School of Medicine, 1-1-1 Hondo, Akita 010-8543, Japan.

PMID: 24194627 PMCID: PMC3813821 DOI: 10.1267/ahc.13007

Abstract

The primary function of hepatic stellate cells (HSCs) is the storage of vitamin A. However, they are also responsible for liver fibrosis and are therapeutic targets for treatment of liver cirrhosis. Among the many molecular markers that define quiescent or activated states of HSCs, the characteristics of type III intermediate filaments are of particular interest. Whereas vimentin and desmin are upregulated in activated HSCs, glial fibrillary acidic protein is downregulated in activated HSCs. The functional differences between vimentin and desmin are poorly understood. By time-course quantifications of several molecular markers for HSC activation, we observed that the expression of vimentin preceded that of desmin during the transdifferentiation of HSCs. The immunoreactivity of vimentin in transdifferentiated HSCs was more intense in perinuclear regions compared to that of desmin. We propose that the delayed expression of desmin following the expression of vimentin and the peripheral localization of desmin compared to vimentin are both related to the more extended phenotype of transdifferentiating HSCs observed in vitro.

Keywords: desmin; hepatic stellate cell; intermediate filament; myofibroblast; vimentin

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