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Southwick PL, Hahn KM, Chao J, et al. A long-wavelength biolabeling reagent based on the oxonol fluorophore. J Fluoresc. 1995;5(2):231-5doi: 10.1007/BF00727544.
Southwick, P. L., Hahn, K. M., Chao, J., Perry, P. L., Wagman, A. S., Wagner, M., & Waggoner, A. S. (1995). A long-wavelength biolabeling reagent based on the oxonol fluorophore. Journal of fluorescence, 5(2), 231-5. https://doi.org/10.1007/BF00727544
Southwick, P L, et al. "A long-wavelength biolabeling reagent based on the oxonol fluorophore." Journal of fluorescence vol. 5,2 (1995): 231-5. doi: https://doi.org/10.1007/BF00727544
Southwick PL, Hahn KM, Chao J, Perry PL, Wagman AS, Wagner M, Waggoner AS. A long-wavelength biolabeling reagent based on the oxonol fluorophore. J Fluoresc. 1995 Jun;5(2):231-5. doi: 10.1007/BF00727544. PMID: 24226669.
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J Fluoresc. 1995 Jun;5(2):231-5. doi: 10.1007/BF00727544.

A long-wavelength biolabeling reagent based on the oxonol fluorophore.

Journal of fluorescence

P L Southwick, K M Hahn, J Chao, P L Perry, A S Wagman, M Wagner, A S Waggoner

Affiliations

  1. Center for Light Microscope Imaging and Biotechnology, Carnegie Mellon University, 4400 5th Avenue, 15213, Pittsburgh, Pennsylvania.

PMID: 24226669 DOI: 10.1007/BF00727544

Abstract

A red fluorescent dye of the oxonol class, bis-[1-(carboxymethyl)barbituric acid-(5)]-pentamethinoxonol, has been synthesized and, in the form of the succinimidyl active ester, has been applied to antibody labeling for application to flow cytometry and to imaging of tissue sections. The new dye, named CMOX (for carboxymethyloxonol), shows maximum excitation at 583 nm and emission at 611 nm, with a quantum yield of 0.2 in aqueous buffer and methanol. Antibodies labeled with the new dye show favorable brightness, photostability, and low levels of nonspecific binding.

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