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Palni LM, Horgan R, Darrall NM, et al. Cytokinin biosynthesis in crown-gall tissue ofVinca rosea : The significance of nucleotides. Planta. 1983;159(1):50-9doi: 10.1007/BF00998814.
Palni, L. M., Horgan, R., Darrall, N. M., Stuchbury, T., & Wareing, P. F. (1983). Cytokinin biosynthesis in crown-gall tissue ofVinca rosea : The significance of nucleotides. Planta, 159(1), 50-9. https://doi.org/10.1007/BF00998814
Palni, L M, et al. "Cytokinin biosynthesis in crown-gall tissue ofVinca rosea : The significance of nucleotides." Planta vol. 159,1 (1983): 50-9. doi: https://doi.org/10.1007/BF00998814
Palni LM, Horgan R, Darrall NM, Stuchbury T, Wareing PF. Cytokinin biosynthesis in crown-gall tissue ofVinca rosea : The significance of nucleotides. Planta. 1983 Jan;159(1):50-9. doi: 10.1007/BF00998814. PMID: 24258086.
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Planta. 1983 Jan;159(1):50-9. doi: 10.1007/BF00998814.

Cytokinin biosynthesis in crown-gall tissue ofVinca rosea : The significance of nucleotides.

Planta

L M Palni, R Horgan, N M Darrall, T Stuchbury, P F Wareing

Affiliations

  1. Department of Botany and Microbiology, University College of Wales, SY23 3DA, Aberystwyth, Dyfed, UK.

PMID: 24258086 DOI: 10.1007/BF00998814

Abstract

When care was taken to minimise the effects of phosphatase activity during extraction ofVinca rosea crown-gall tumour tissue, a large proportion of extractable cytolinin activity was present in the nucleotide fraction. Analysis using ion-exchange chromatography followed by enzymic or chemical degradation and subsequent identification of the biologically active material indicated that this activity was due to zeatin riboside 5'-monophosphate. This was also the major radiolabelled cytokinin formed when this tissue was supplied with [(14)C]adenine. The incorporation of radioactivity from [(14)C]adenosine into free cytokinins was also shown, but no incorporation of radioactivity was found when [(3)H]mevalonic acid lactone was supplied to this tissue under the same conditions. In parallel experiments using normal stem callus tissue ofV. rosea, no incorporation of [(14)C]adenine into free cytokinins was observed. The significance of these results is discussed in relation to a possible transfer-RNA-independent pathway of cytokinin biosynthesis, operating primarily at the mononucleotide level.

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