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Boocock MR, Rice PA. A proposed mechanism for IS607-family serine transposases. Mob DNA. 2013;4(1):24doi: 10.1186/1759-8753-4-24.
Boocock, M. R., & Rice, P. A. (2013). A proposed mechanism for IS607-family serine transposases. Mobile DNA, 4(1), 24. https://doi.org/10.1186/1759-8753-4-24
Boocock, Martin R, and Rice, Phoebe A. "A proposed mechanism for IS607-family serine transposases." Mobile DNA vol. 4,1 (2013): 24. doi: https://doi.org/10.1186/1759-8753-4-24
Boocock MR, Rice PA. A proposed mechanism for IS607-family serine transposases. Mob DNA. 2013 Nov 06;4(1):24. doi: 10.1186/1759-8753-4-24. PMID: 24195768; PMCID: PMC4058570.
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Mob DNA. 2013 Nov 06;4(1):24. doi: 10.1186/1759-8753-4-24.

A proposed mechanism for IS607-family serine transposases.

Mobile DNA

Martin R Boocock, Phoebe A Rice

Affiliations

  1. Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, IL 60637, USA. [email protected].

PMID: 24195768 PMCID: PMC4058570 DOI: 10.1186/1759-8753-4-24

Abstract

BACKGROUND: The transposases encoded by the IS607 family of mobile elements are unusual serine recombinases with an inverted domain order and minimal specificity for target DNA.

RESULTS: Structural genomics groups have determined three crystal structures of the catalytic domains of IS607 family transposases. The dimers formed by these catalytic domains are very different from those seen for other serine recombinases and include interactions that usually only occur upon formation of a synaptic tetramer.

CONCLUSIONS: Based on these structures, we propose a model for how IS607-family transposases could form a synaptic tetramer. The model suggests that, unlike other serine recombinases, these enzymes carry out sequence-specific DNA binding and catalysis in trans: the DNA binding and catalytic domains of each subunit are proposed to interact with different DNA duplexes. The model also suggests an explanation for the minimal target DNA specificity.

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