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Schmidt T, Boblenz K, Metzlaff M, et al. DNA fingerprinting in sugar beet (Beta vulgaris) - identification of double-haploid breeding lines. Theor Appl Genet. 1993;85(6):653-7doi: 10.1007/BF00225001.
Schmidt, T., Boblenz, K., Metzlaff, M., Kaemmer, D., Weising, K., & Kahl, G. (1993). DNA fingerprinting in sugar beet (Beta vulgaris) - identification of double-haploid breeding lines. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 85(6), 653-7. https://doi.org/10.1007/BF00225001
Schmidt, T, et al. "DNA fingerprinting in sugar beet (Beta vulgaris) - identification of double-haploid breeding lines." TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik vol. 85,6 (1993): 653-7. doi: https://doi.org/10.1007/BF00225001
Schmidt T, Boblenz K, Metzlaff M, Kaemmer D, Weising K, Kahl G. DNA fingerprinting in sugar beet (Beta vulgaris) - identification of double-haploid breeding lines. Theor Appl Genet. 1993 Feb;85(6):653-7. doi: 10.1007/BF00225001. PMID: 24196032.
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Theor Appl Genet. 1993 Feb;85(6):653-7. doi: 10.1007/BF00225001.

DNA fingerprinting in sugar beet (Beta vulgaris) - identification of double-haploid breeding lines.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

T Schmidt, K Boblenz, M Metzlaff, D Kaemmer, K Weising, G Kahl

Affiliations

  1. Institute of Genetics, Martin-Luther-University, Domplatz 1, 4020, Halle, Germany.

PMID: 24196032 DOI: 10.1007/BF00225001

Abstract

The distribution and abundance of simple repetitive sequences complementary to the synthetic oligonucleotides (GACA)4, (GATA)4, (GTG)5 and (CA)8 in the genomes of several cultivars of Beta vulgaris and in the wild beet B. vulgaris ssp. maritima were investigated. Hybridization experiments revealed that all four motifs were present, though at different abundances, in the genomes of all of the investigated beet cultivars. Considerable intraspecific variation of the resulting DNA fingerprints was observed. The extent of polymorphism depends on the oligonucleotide probe. The most informative banding patterns were obtained with the (GATA)4 probe hybridized to HinfI-, HaeIII-, or RsaI-restricted DNA, respectively. DNA fingerprinting with (GATA)4 allowed a clear differentiation of double-haploid breeding lines (DH lines). We demonstrated that the application of oligonucleotide probes for DNA fingerprinting is a sensitive tool for genome diagnosis in cultivated beet.

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