Display options
Cite
Chibbar RN, Kartha KK, Datla RS, et al. The effect of different promoter-sequences on transient expression of gus reporter gene in cultured barley (Hordeum vulgare L.) cells. Plant Cell Rep. 1993;12(9):506-9doi: 10.1007/BF00236096.
Chibbar, R. N., Kartha, K. K., Datla, R. S., Leung, N., Caswell, K., Mallard, C. S., & Steinhauer, L. (1993). The effect of different promoter-sequences on transient expression of gus reporter gene in cultured barley (Hordeum vulgare L.) cells. Plant cell reports, 12(9), 506-9. https://doi.org/10.1007/BF00236096
Chibbar, R N, et al. "The effect of different promoter-sequences on transient expression of gus reporter gene in cultured barley (Hordeum vulgare L.) cells." Plant cell reports vol. 12,9 (1993): 506-9. doi: https://doi.org/10.1007/BF00236096
Chibbar RN, Kartha KK, Datla RS, Leung N, Caswell K, Mallard CS, Steinhauer L. The effect of different promoter-sequences on transient expression of gus reporter gene in cultured barley (Hordeum vulgare L.) cells. Plant Cell Rep. 1993 Jul;12(9):506-9. doi: 10.1007/BF00236096. PMID: 24196110.
Copy Download .nbib Format: NLM
Share it on

Plant Cell Rep. 1993 Jul;12(9):506-9. doi: 10.1007/BF00236096.

The effect of different promoter-sequences on transient expression of gus reporter gene in cultured barley (Hordeum vulgare L.) cells.

Plant cell reports

R N Chibbar, K K Kartha, R S Datla, N Leung, K Caswell, C S Mallard, L Steinhauer

Affiliations

  1. Plant Biotechnology Institute, National Research Council Canada, 110 Gymnasium Place, S7N 0W9, Saskatoon, Saskatchewan, Canada.

PMID: 24196110 DOI: 10.1007/BF00236096

Abstract

The cauliflower mosaic virus 35S (35S) and the enhanced 35S (E35S) promoters fused with maize alcohol dehydrogenase (Adh1) intron1 or maize shrunken locus (sh1) intronl along with maize Adh1 and rice actin (Act1) promoters fused to their respective first introns were tested for transient expression of the E.coli β-glucuronidase (gus) reporter gene in cultured barley (Hordeum vulgare L) cells. The plasmids, carrying the respective promoterintron combinations to drive the gus fused to nopaline synthase (nos) terminator, were introduced into cultured barley cells using a particle gun. The rice Act1 promoter with its first intron gave the highest expression of all promoter intron combinations studied. This was followed by the E35S promoter and no significant differences were observed between the other two promoters tested. The rice actin promoter is now being used to drive selectable marker genes to obtain stably transformed cereal cells.

References

  1. Genes Dev. 1987 Dec;1(10):1183-200 - PubMed
  2. Anal Biochem. 1976 May 7;72:248-54 - PubMed
  3. Plant Cell. 1990 Feb;2(2):163-71 - PubMed
  4. Plant Physiol. 1988 Dec;88(4):1063-6 - PubMed
  5. Theor Appl Genet. 1991 Apr;81(4):437-44 - PubMed
  6. Plant Physiol. 1989 Dec;91(4):1575-9 - PubMed
  7. Plant Cell. 1989 Jan;1(1):141-50 - PubMed
  8. Theor Appl Genet. 1990 May;79(3):353-9 - PubMed
  9. Nature. 1985 Feb 28-Mar 6;313(6005):810-2 - PubMed
  10. Cell. 1982 Oct;30(3):763-73 - PubMed
  11. Mol Gen Genet. 1991 Dec;231(1):150-60 - PubMed
  12. Science. 1987 Jun 5;236(4806):1299-302 - PubMed
  13. Gene. 1991 May 30;101(2):239-46 - PubMed
  14. Plant Cell Rep. 1989 Dec;8(8):429-32 - PubMed
  15. Plant Cell. 1990 Jul;2(7):603-618 - PubMed
  16. Plant Cell Rep. 1990 Jun;9(1):1-5 - PubMed
  17. Theor Appl Genet. 1988 Dec;76(6):835-40 - PubMed
  18. Biotechnology (N Y). 1990 Sep;8(9):833-9 - PubMed

Publication Types