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Wilms FH, Wolters-Arts AM, Derksen J. Orientation of cellulose microfibrils in cortical cells of tobacco explants : Effects of microtubule-depolymerizing drugs. Planta. 1990;182(1):1-8doi: 10.1007/BF00239976.
Wilms, F. H., Wolters-Arts, A. M., & Derksen, J. (1990). Orientation of cellulose microfibrils in cortical cells of tobacco explants : Effects of microtubule-depolymerizing drugs. Planta, 182(1), 1-8. https://doi.org/10.1007/BF00239976
Wilms, F H, et al. "Orientation of cellulose microfibrils in cortical cells of tobacco explants : Effects of microtubule-depolymerizing drugs." Planta vol. 182,1 (1990): 1-8. doi: https://doi.org/10.1007/BF00239976
Wilms FH, Wolters-Arts AM, Derksen J. Orientation of cellulose microfibrils in cortical cells of tobacco explants : Effects of microtubule-depolymerizing drugs. Planta. 1990 Aug;182(1):1-8. doi: 10.1007/BF00239976. PMID: 24196992.
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Planta. 1990 Aug;182(1):1-8. doi: 10.1007/BF00239976.

Orientation of cellulose microfibrils in cortical cells of tobacco explants : Effects of microtubule-depolymerizing drugs.

Planta

F H Wilms, A M Wolters-Arts, J Derksen

Affiliations

  1. Department of Experimental Botany, University of Nijmegen, Toernooiveld, NL-6525 ED, Nijmegen, The Netherlands.

PMID: 24196992 DOI: 10.1007/BF00239976

Abstract

The deposition of nascent cellulose microfibrils (CMFs) was studied in the walls of cortical cells in explants of Nicotiana tabacum L. flower stalks. In freshly cut explants the CMFs were deposited in two distinct and alternating orientations - all given with respect to the longitudinal axis of the cell -, at 75° and 115°, in a left-handed (S-helix) and right-handed (Z-helix) form, respectively. The CMFs deposited in these orientations did not form uninterrupted layers, but sheets in which both orientations were present. After explantation, the synthesis of CMFs and their deposition in bundles continued. New orientations occurred within 6 h. After 6 h a new sheet was deposited, with orientations of 15° (S-helix) and 165° (Z-helix). The changes could be seen as sudden bends in individual CMFs or in small bundles of CMFs. In the next stage, more CMFs were deposited with these new orientations and the bundles became larger. New orientations arose by a shift towards more longitudinal directions, starting from either the S-helix or the Z-helix form. It was only after an almost longitudinal orientation was reached that the CMFs were deposited in two opposing directions again and a new sheet was formed. Neither colchicine nor cremart influenced the changes in CMF deposition. It is concluded that microtubules do not control CMF deposition in cortical cells of tobacco explants; control of CMF deposition and microtubule orientation occurs by factors related to cell polarity.

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