Display options
Cite
Natali L, Giordani T, Cionini G, et al. Heterochromatin and repetitive DNA frequency variation in regenerated plants of Helianthus annuus L. Theor Appl Genet. 1995;91(3):395-400doi: 10.1007/BF00222965.
Natali, L., Giordani, T., Cionini, G., Pugliesi, C., Fambrini, M., & Cavallini, A. (1995). Heterochromatin and repetitive DNA frequency variation in regenerated plants of Helianthus annuus L. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 91(3), 395-400. https://doi.org/10.1007/BF00222965
Natali, L, et al. "Heterochromatin and repetitive DNA frequency variation in regenerated plants of Helianthus annuus L." TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik vol. 91,3 (1995): 395-400. doi: https://doi.org/10.1007/BF00222965
Natali L, Giordani T, Cionini G, Pugliesi C, Fambrini M, Cavallini A. Heterochromatin and repetitive DNA frequency variation in regenerated plants of Helianthus annuus L. Theor Appl Genet. 1995 Aug;91(3):395-400. doi: 10.1007/BF00222965. PMID: 24169827.
Copy Download .nbib Format: NLM
Share it on

Theor Appl Genet. 1995 Aug;91(3):395-400. doi: 10.1007/BF00222965.

Heterochromatin and repetitive DNA frequency variation in regenerated plants of Helianthus annuus L.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

L Natali, T Giordani, G Cionini, C Pugliesi, M Fambrini, A Cavallini

Affiliations

  1. Genetics Section, Department of Agricultural Plant Biology of the University, Via Matteotti 1/B, I-56124, Pisa, Italy.

PMID: 24169827 DOI: 10.1007/BF00222965

Abstract

Plant regeneration from cotyledons of seeds of a single progeny of a pure line of Helianthus annuus was studied in respect of the nuclear DNA contents of control and regenerated plants. Control plants were divided into two groups: those developed from seeds at the periphery of the inflorescence (showing a high basic 4C DNA content) and those from seeds developed in the middle of the inflorescence (showing a low basic 4C DNA content). It was observed that plants from peripheral seeds have a higher morphogenetic potential than those from central seeds. Cytophotometric analyses indicated that plants regenerated from cotyledons of both peripheral and central seeds show the same basic 4C DNA amount, which is higher that that observed in vivo in peripheral seeds. Molecular analysis by slot blotting and hybridization with different DNA families showed that the difference in nuclear DNA content between plants from peripheral and central seeds in vivo are mainly related to differences in the frequency of highly repeated, "slow" medium repeated (MR2), and ribosomal DNA families; by contrast, the increase in DNA amount in regenerated plants is mainly due to "fast" medium repeated sequences (MR1). Moreover, the frequency of kinetically isolated "unique" sequences was higher in peripheral seeds than in central ones and still higher in regenerated plants. Optical-density measurements of interphase nuclei showed an increase of heterochromatin in regenerated plants, suggesting that, whatever DNA is amplified in these plants, it remains condensed and probably inactive.

References

  1. Genetics. 1991 Jul;128(3):619-30 - PubMed
  2. Nucleic Acids Res. 1984 May 11;12(9):3983-4000 - PubMed
  3. Theor Appl Genet. 1993 Jan;85(5):506-12 - PubMed
  4. Mol Gen Genet. 1987 Dec;210(3):373-80 - PubMed
  5. Theor Appl Genet. 1991 Feb;81(2):227-32 - PubMed
  6. Cell Differ. 1986 May;18(3):157-61 - PubMed
  7. Methods Enzymol. 1974;29:363-418 - PubMed
  8. Theor Appl Genet. 1985 Dec;71(3):500-5 - PubMed
  9. Genome. 1993 Oct;36(5):906-12 - PubMed
  10. Theor Appl Genet. 1994 Jul;88(5):557-60 - PubMed
  11. Theor Appl Genet. 1981 Oct;60(4):197-214 - PubMed
  12. Theor Appl Genet. 1986 Nov;73(1):20-6 - PubMed
  13. Plant Mol Biol. 1992 Apr;18(6):1073-82 - PubMed
  14. Theor Appl Genet. 1992 Sep;84(7-8):874-9 - PubMed
  15. Theor Appl Genet. 1989 Jan;77(1):12-6 - PubMed
  16. Proc R Soc Lond B Biol Sci. 1972 Jun 6;181(1063):109-35 - PubMed
  17. Theor Appl Genet. 1986 Jan;71(4):637-43 - PubMed

Publication Types