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Heath DW, Earle ED. Synthesis of high erucic acid rapeseed (Brassica napus L.) somatic hybrids with improved agronomic characters. Theor Appl Genet. 1995;91(6):1129-36doi: 10.1007/BF00223931.
Heath, D. W., & Earle, E. D. (1995). Synthesis of high erucic acid rapeseed (Brassica napus L.) somatic hybrids with improved agronomic characters. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 91(6), 1129-36. https://doi.org/10.1007/BF00223931
Heath, D W, and Earle, E D. "Synthesis of high erucic acid rapeseed (Brassica napus L.) somatic hybrids with improved agronomic characters." TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik vol. 91,6 (1995): 1129-36. doi: https://doi.org/10.1007/BF00223931
Heath DW, Earle ED. Synthesis of high erucic acid rapeseed (Brassica napus L.) somatic hybrids with improved agronomic characters. Theor Appl Genet. 1995 Nov;91(6):1129-36. doi: 10.1007/BF00223931. PMID: 24170008.
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Theor Appl Genet. 1995 Nov;91(6):1129-36. doi: 10.1007/BF00223931.

Synthesis of high erucic acid rapeseed (Brassica napus L.) somatic hybrids with improved agronomic characters.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

D W Heath, E D Earle

Affiliations

  1. Department of Plant Breeding and Biometry, Cornell University, 14853-1902, Ithaca, NY, USA.

PMID: 24170008 DOI: 10.1007/BF00223931

Abstract

Novel Brassica napus somatic hybrids have been created through protoplast fusion of B. oleracea var. botrytis and B. rapa var. oleifera genotypes selected for high erucic acid (22:1) content in the seed oil. Fifty amphidiploids (aacc) and one putative hexaploid (aacccc) hybrid were recovered in one fusion experiment. Conversely, only one amphidiploid and numerous regenerates with higher DNA contents were produced in a similar fusion using a different B. rapa partner. Hybridity was confirmed by morphology, isozyme expression, flow cytometry, and DNA hybridization. Analysis of organellar DNA revealed a distinct bias toward the inheritance of chloroplasts from the B. rapa (aa) genome. All amphidiploids set self-pollinated seed. A erucic acid content as high as 57.4% was found in the seed oil of one regenerated plant. Fatty acid composition was stable in the R1 generation and was coupled with increased female fertility. Other novel agronomic characters in the hybrids recovered include large seed size, lodging resistance, and non-shattering seed pods.

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