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Higgs DC, Colbert JT. β-glucuronidase gene expression and mRNA stability in oat protoplasts. Plant Cell Rep. 1993;12(7):445-52doi: 10.1007/BF00234710.
Higgs, D. C., & Colbert, J. T. (1993). β-glucuronidase gene expression and mRNA stability in oat protoplasts. Plant cell reports, 12(7), 445-52. https://doi.org/10.1007/BF00234710
Higgs, D C, and Colbert, J T. "β-glucuronidase gene expression and mRNA stability in oat protoplasts." Plant cell reports vol. 12,7 (1993): 445-52. doi: https://doi.org/10.1007/BF00234710
Higgs DC, Colbert JT. β-glucuronidase gene expression and mRNA stability in oat protoplasts. Plant Cell Rep. 1993 May;12(7):445-52. doi: 10.1007/BF00234710. PMID: 24197350.
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Plant Cell Rep. 1993 May;12(7):445-52. doi: 10.1007/BF00234710.

β-glucuronidase gene expression and mRNA stability in oat protoplasts.

Plant cell reports

D C Higgs, J T Colbert

Affiliations

  1. Department of Botany and Molecular, Cellular, and Developmental Biology Program, Iowa State University, IA 50011, Ames, USA.

PMID: 24197350 DOI: 10.1007/BF00234710

Abstract

Protoplasts derived from oat (Avena sativa L.) suspension culture cells (7 days after subculturing) were electroporated with plasmid DNA containing the Escherichia coli uidA gene encoding the ß-glucuronidase reporter enzyme. Consistently high enzyme activity was observed with electroporation conditions of 500 μF and 1125 volts/cm. Enzyme activity and mRNA accumulation time courses were determined. The maximum enzyme activity was detected at 24 hours after electroporation, while the maximum mRNA level was detected at 12 hours after electroporation. ß-glucuronidase mRNA was in vitro synthesized with and without a 5' methylated cap and then electroporated into protoplasts. Only capped mRNA produced significant enzyme activity. By electroporating radiolabeled, in vitro synthesized mRNA, the ß-glucuronidase mRNA half-life was estimated to be ∼35 minutes in oat protoplasts.

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