Nanotechnol Sci Appl. 2008 Sep 19;1:33-44. eCollection 2008.

Vibrio fischeri and Escherichia coli adhesion tendencies towards photolithographically modified nanosmooth poly (tert-butyl methacrylate) polymer surfaces.

Nanotechnology, science and applications

Elena P Ivanova, Natasa Mitik-Dineva, Radu C Mocanasu, Sarah Murphy, James Wang, Grant van Riessen, Russell J Crawford

PMID: 24198459 PMCID: PMC3781764

Abstract

This study reports the adhesion behavior of two bacterial species, Vibrio fischeri and Escherichia coli, to the photoresistant poly(tert-butyl methacrylate) (P(tBMA)) polymer surface. The data has demonstrated that ultraviolet irradiation of P(tBMA) was able to provide control over bacterial adhesion tendencies. Following photolithography, several of the surface characteristics of P(tBMA) were found to be altered. Atomic force microscopy analysis indicated that photolithographically modified P(tBMA) (henceforth termed 'modified polymer') appeared as a 'nanosmooth' surface with an average surface roughness of 1.6 nm. Although confocal laser scanning microscopy and scanning electron microscopy analysis clearly demonstrated that V. fischeri and E. coli presented largely different patterns of attachment in order to adhere to the same surfaces, both species exhibited a greater adhesion propensity towards the 'nanosmooth' surface. The adhesion of both species to the modified polymer surface appeared to be facilitated by an elevated production of extracellular polymeric substances when in contact with the substrate.

Keywords: bacterial attachment; extracellular polymeric substances; poly(tert-butylmethacrylate) polymeric surfaces; surface nanotopography

References

1. Annu Rev Microbiol. 2002;56:187-209 - PubMed
2. Infect Immun. 1990 Jun;58(6):1769-73 - PubMed
3. Biomed Mater. 2006 Mar;1(1):24-32 - PubMed
4. Microb Ecol. 1989 Jan;17(1):1-15 - PubMed
5. Biochemistry. 1965 May;4:876-83 - PubMed
6. J Bacteriol. 1935 Mar;29(3):239-51 - PubMed
7. J Chromatogr B Biomed Sci Appl. 1999 Jan 8;721(1):31-7 - PubMed
8. Colloids Surf B Biointerfaces. 2004 Jul 15;36(2):81-90 - PubMed
9. Emerg Infect Dis. 2002 Sep;8(9):881-90 - PubMed
10. Proc Natl Acad Sci U S A. 2000 Nov 21;97(24):13092-6 - PubMed
11. Langmuir. 2005 Jul 5;21(14):6462-72 - PubMed
12. Adv Dent Res. 1997 Apr;11(1):24-32 - PubMed
13. Infect Immun. 1983 Mar;39(3):1285-90 - PubMed
14. Biomaterials. 1991 Nov;12(9):853-60 - PubMed
15. Appl Microbiol Biotechnol. 2006 Dec;73(3):669-75 - PubMed
16. Water Sci Technol. 2001;43(6):1-8 - PubMed
17. Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7907-12 - PubMed
18. Biophys Chem. 1995 Aug;55(3):273-7 - PubMed
19. Biomaterials. 1988 May;9(3):285-9 - PubMed
20. J Biomed Mater Res. 2000 May;50(2):160-70 - PubMed
21. Mol Microbiol. 1999 Nov;34(3):586-95 - PubMed
22. Microbiology (Reading). 2002 Jun;148(Pt 6):1863-1870 - PubMed
23. Nat Rev Microbiol. 2007 Mar;5(3):209-18 - PubMed
24. Gastrointest Endosc. 1993 May-Jun;39(3):422-5 - PubMed
25. J Gen Microbiol. 1985 Sep;131(9):2485-91 - PubMed
26. Appl Environ Microbiol. 1986 Jun;51(6):1321-5 - PubMed
27. Int Microbiol. 2006 Mar;9(1):37-46 - PubMed
28. Biomaterials. 1989 Jul;10(5):325-8 - PubMed
29. Langmuir. 2005 Dec 20;21(26):12315-9 - PubMed
30. J Biomed Mater Res. 1998 Fall;43(3):338-48 - PubMed
31. Microbiol Mol Biol Rev. 2000 Dec;64(4):847-67 - PubMed
32. Proc Natl Acad Sci U S A. 1999 Mar 30;96(7):4028-33 - PubMed
33. J Gen Microbiol. 1983 Sep;129(9):2959-68 - PubMed
34. Water Sci Technol. 2001;43(6):135-41 - PubMed
35. J Bacteriol. 1943 Jul;46(1):39-56 - PubMed
36. J Colloid Interface Sci. 2004 Nov 15;279(2):410-7 - PubMed
37. Biomacromolecules. 2005 Sep-Oct;6(5):2427-48 - PubMed
38. Int J Artif Organs. 1993 Nov;16(11):755-63 - PubMed
39. Water Sci Technol. 2001;43(6):77-86 - PubMed
40. J Gen Microbiol. 1983 Feb;129(2):439-45 - PubMed
41. Adv Dent Res. 1997 Apr;11(1):176-85 - PubMed

Publication Types

• Journal Article