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Weber G, de Groot E, Schweiger HG. Synchronization of protoplasts from Glycine max (L.) Merr. and Brassica napus (L.). Planta. 1986;168(2):273-80doi: 10.1007/BF00402974.
Weber, G., de Groot, E., & Schweiger, H. G. (1986). Synchronization of protoplasts from Glycine max (L.) Merr. and Brassica napus (L.). Planta, 168(2), 273-80. https://doi.org/10.1007/BF00402974
Weber, G, et al. "Synchronization of protoplasts from Glycine max (L.) Merr. and Brassica napus (L.)." Planta vol. 168,2 (1986): 273-80. doi: https://doi.org/10.1007/BF00402974
Weber G, de Groot E, Schweiger HG. Synchronization of protoplasts from Glycine max (L.) Merr. and Brassica napus (L.). Planta. 1986 Jun;168(2):273-80. doi: 10.1007/BF00402974. PMID: 24232032.
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Planta. 1986 Jun;168(2):273-80. doi: 10.1007/BF00402974.

Synchronization of protoplasts from Glycine max (L.) Merr. and Brassica napus (L.).

Planta

G Weber, E de Groot, H G Schweiger

Affiliations

  1. Max-Planck-Institut für Zellbiologie, Rosenhof, D-6802, Ladenburg, Federal Republic of Germany.

PMID: 24232032 DOI: 10.1007/BF00402974

Abstract

Cells of Glycine max originating in a suspension culture and cells of Brassica napus prepared from hypocotyls were synchronized. Synchronization was achieved by preparing protoplasts in the usual way and subsequently letting the protoplasts regenerate into cells by removing the cell-wall-digesting enzymes. More than 70% of the cells had divided synchronously at the end of the first cycle as determined by the mitotic index. The high frequency of mitosis critically depended on the osmolality of the medium. The duration of the S-phase was estimated by measuring the activity of thymidylate kinase as well as incorporation of [(3)H]deoxythymidine into acid-insoluble material. The data indicate that synchronization is induced by resetting the cell cycle.

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