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Microb Ecol. 1977 Sep;4(3):267-77. doi: 10.1007/BF02015083.

Bacterioneuston examined with critical point drying and transmission electron microscopy.

Microbial ecology

L Y Young

Affiliations

  1. Environmental Engineering and Science, Department of Civil Engineering, Stanford University, 94305, Stanford, California.

PMID: 24232166 DOI: 10.1007/BF02015083

Abstract

Morphological details of bacteria collected at the air/seawater interface are greatly enhanced when transmission electron microscopy is coupled with critical point dried preparations. Few artifacts and minimal distortion permit a more accurate appraisal of an organism's cell characteristics and its relationship to its microenvironment. Cell surface and extracellular polymer materials are clearly distinguished in a manner not feasible with other preparations.

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