Display options
Cite
Fukuda N, Honda S. Development of growth selection systems to isolate a-type or α-type of yeast cells spontaneously emerging from MATa/α diploids. J Biol Eng. 2013;7(1):27doi: 10.1186/1754-1611-7-27.
Fukuda, N., & Honda, S. (2013). Development of growth selection systems to isolate a-type or α-type of yeast cells spontaneously emerging from MATa/α diploids. Journal of biological engineering, 7(1), 27. https://doi.org/10.1186/1754-1611-7-27
Fukuda, Nobuo, and Honda, Shinya. "Development of growth selection systems to isolate a-type or α-type of yeast cells spontaneously emerging from MATa/α diploids." Journal of biological engineering vol. 7,1 (2013): 27. doi: https://doi.org/10.1186/1754-1611-7-27
Fukuda N, Honda S. Development of growth selection systems to isolate a-type or α-type of yeast cells spontaneously emerging from MATa/α diploids. J Biol Eng. 2013 Nov 21;7(1):27. doi: 10.1186/1754-1611-7-27. PMID: 24261936; PMCID: PMC3923440.
Copy Download .nbib Format: NLM
Share it on

J Biol Eng. 2013 Nov 21;7(1):27. doi: 10.1186/1754-1611-7-27.

Development of growth selection systems to isolate a-type or α-type of yeast cells spontaneously emerging from MATa/α diploids.

Journal of biological engineering

Nobuo Fukuda, Shinya Honda

Affiliations

  1. Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Higashi, Tsukuba, Ibaraki 305-8566, Japan. [email protected].

PMID: 24261936 PMCID: PMC3923440 DOI: 10.1186/1754-1611-7-27

Abstract

BACKGROUND: Manufacture of MATa and MATα yeast cells is required for crossbreeding, a procedure that permits hybridization and the generation of new heterozygous strains. Crossbreeding also can be performed with a- and α-type of cells, which have the same mating abilities as MATa and MATα haploid cells, respectively.

RESULTS: In this work, we describe a method to generate a- and α-type of cells via the naturally-occurring chromosomal aberration in parental MATa/α diploids. We successfully designed suitable genetic circuits for expression of the URA3 selection marker gene to permit isolation of a- and α-type of cells, respectively, on solid medium lacking uracil. Furthermore we succeeded in generation of zygotes by mating of both the manufactured a- and α-type of yeast cells.

CONCLUSIONS: This process does not require exposure to mutagens such as UV irradiation, thereby avoiding the accumulation of undesirable mutations that would detract from the valuable traits that are under study. All the genetic modifications in the current study were introduced into yeast cells using plasmids, meaning that these traits can be removed without altering the genome sequence. This approach provides a reliable and versatile tool for scientific research and industrial yeast crossbreeding.

References

  1. Genetics. 2004 Feb;166(2):653-60 - PubMed
  2. Acta Microbiol Immunol Hung. 2002;49(4):483-91 - PubMed
  3. Mol Cell Biol. 1991 Oct;11(10):5372-80 - PubMed
  4. FEBS J. 2010 Apr;277(7):1704-12 - PubMed
  5. Nucleic Acids Res. 1992 Mar 25;20(6):1425 - PubMed
  6. Mol Cell Biol. 2011 Apr;31(8):1701-9 - PubMed
  7. Yeast. 1997 Feb;13(2):101-17 - PubMed
  8. Yeast. 1998 Jan 30;14(2):115-32 - PubMed
  9. Appl Environ Microbiol. 2001 Sep;67(9):4346-8 - PubMed
  10. Genetics. 2008 Jul;179(3):1179-95 - PubMed
  11. Genetics. 2000 Dec;156(4):1531-48 - PubMed
  12. Mutat Res. 1990 Aug;231(2):177-86 - PubMed
  13. ACS Synth Biol. 2013 Dec 20;2(12):697-704 - PubMed
  14. Mutat Res. 2004 Nov 22;556(1-2):183-91 - PubMed
  15. Mutat Res. 1990 Jul;241(3):225-42 - PubMed
  16. PLoS One. 2013 Jul 10;8(7):e68094 - PubMed
  17. Appl Microbiol Biotechnol. 2008 Jan;77(5):1073-82 - PubMed
  18. Yeast. 2006 Oct-Nov;23(14-15):1097-106 - PubMed
  19. Appl Microbiol Biotechnol. 2006 Feb;69(6):689-96 - PubMed
  20. FEBS J. 2011 Sep;278(17):3086-94 - PubMed

Publication Types