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Kyozuka J, Otoo E, Shimamoto K. Plant regeneration from protoplasts of indica rice: genotypic differences in culture response. Theor Appl Genet. 1988;76(6):887-90doi: 10.1007/BF00273677.
Kyozuka, J., Otoo, E., & Shimamoto, K. (1988). Plant regeneration from protoplasts of indica rice: genotypic differences in culture response. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 76(6), 887-90. https://doi.org/10.1007/BF00273677
Kyozuka, J, et al. "Plant regeneration from protoplasts of indica rice: genotypic differences in culture response." TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik vol. 76,6 (1988): 887-90. doi: https://doi.org/10.1007/BF00273677
Kyozuka J, Otoo E, Shimamoto K. Plant regeneration from protoplasts of indica rice: genotypic differences in culture response. Theor Appl Genet. 1988 Dec;76(6):887-90. doi: 10.1007/BF00273677. PMID: 24232400.
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Theor Appl Genet. 1988 Dec;76(6):887-90. doi: 10.1007/BF00273677.

Plant regeneration from protoplasts of indica rice: genotypic differences in culture response.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

J Kyozuka, E Otoo, K Shimamoto

Affiliations

  1. Plantech Research Institute, 1000 Kamoshida, Midori-ku, 227, Yokohama, Japan.

PMID: 24232400 DOI: 10.1007/BF00273677

Abstract

Fourteen varieties of indica rice (Oryza saliva L.) were examined for their capacity for plant regeneration from protoplasts using the nurse culture methods developed for japonica rice. Calli induced from germinating seeds were grouped into two types: type I, white and compact; type II, yellow and friable. In four varieties producing type II callus, colony formation (2%-4.5%) and plant regeneration (2%-35%) were observed. The inability to develop suspension cultures was a major obstacle in regenerating plants from protoplasts of the remaining rice varieties studied.

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