Display options
Cite
Kooijman R, Elzenga TJ, de Wildt P, et al. Light dependence of sexual agglutinability in Chlamydomonas eugametos. Planta. 1986;169(3):370-8doi: 10.1007/BF00392133.
Kooijman, R., Elzenga, T. J., de Wildt, P., Musgrave, A., Schuring, F., & van den Ende, H. (1986). Light dependence of sexual agglutinability in Chlamydomonas eugametos. Planta, 169(3), 370-8. https://doi.org/10.1007/BF00392133
Kooijman, R, et al. "Light dependence of sexual agglutinability in Chlamydomonas eugametos." Planta vol. 169,3 (1986): 370-8. doi: https://doi.org/10.1007/BF00392133
Kooijman R, Elzenga TJ, de Wildt P, Musgrave A, Schuring F, van den Ende H. Light dependence of sexual agglutinability in Chlamydomonas eugametos. Planta. 1986 Nov;169(3):370-8. doi: 10.1007/BF00392133. PMID: 24232649.
Copy Download .nbib Format: NLM
Share it on

Planta. 1986 Nov;169(3):370-8. doi: 10.1007/BF00392133.

Light dependence of sexual agglutinability in Chlamydomonas eugametos.

Planta

R Kooijman, T J Elzenga, P de Wildt, A Musgrave, F Schuring, H van den Ende

Affiliations

  1. Department of Plant Physiology, University of Amsterdam, Kruislaan 318, 1098 SM, Amsterdam, The Netherlands.

PMID: 24232649 DOI: 10.1007/BF00392133

Abstract

The mating activity of mating-type plus gametes of Chlamydomonas eugametos depends on light. Cells lost their ability to agglutinate with mating-type minus gametes after a dark period of 30 min. They regained their agglutinability after 10 min exposure to light. Other mating reactions, such as tipping and flagellar tip activation, were not dependent upon light. Since cycloheximide and tunicamycin did not affect the light-induced activation of flagellar agglutinability, no protein synthesis or glycosylation is involved in this process. Equal amounts of biologically active agglutination factor could be extracted from cells placed either in light or in darkness. A minor portion of the active material was found to be located on the flagellar surface of illuminated cells. No active material was found on the flagellar surface of dark-exposed cells, whereas their cell bodies contained the same amount of active material as the cell bodies of illuminated cells. Since a light-induced flow of agglutination factors from the cell body to the flagella could not be detected and dark-exposed cells could be slightly activated by amputation or fixation by glutaraldehyde, we propose that light affects flagellar agglutinability by an in-situ modification of the agglutination factor on the flagella. When mt (+) and mt (-) strains were crossed and the progeny examined for light-sensitivity, it was apparent that this phenomenon is not mating type-linked.

References

  1. Plant Physiol. 1975 May;55(5):815-21 - PubMed
  2. Plant Physiol. 1985 Nov;79(3):740-5 - PubMed
  3. Dev Biol. 1975 Apr;43(2):264-76 - PubMed
  4. Proc Natl Acad Sci U S A. 1983 Oct;80(19):5898-901 - PubMed
  5. Planta. 1986 Apr;167(4):544-53 - PubMed
  6. Biochim Biophys Acta. 1975 Sep 9;405(1):53-61 - PubMed
  7. Planta. 1979 Jan;145(5):417-25 - PubMed
  8. J Gen Physiol. 1954 Jul 20;37(6):729-42 - PubMed
  9. J Cell Biol. 1978 Dec;79(3):680-93 - PubMed
  10. J Cell Biol. 1977 Jan;72(1):133-43 - PubMed
  11. J Cell Biol. 1980 Jan;84(1):203-10 - PubMed
  12. Exp Cell Res. 1982 Oct;141(2):245-52 - PubMed
  13. Plant Physiol. 1973 Jun;51(6):1046-50 - PubMed
  14. J Gen Microbiol. 1956 Aug;15(1):170-85 - PubMed
  15. Exp Cell Res. 1984 Jun;152(2):322-30 - PubMed
  16. Planta. 1985 Oct;166(2):234-43 - PubMed
  17. Arch Microbiol. 1976 Aug;109(1-2):31-5 - PubMed
  18. Nature. 1986 Mar 6-12;320(6057):67-70 - PubMed
  19. Planta. 1981 Dec;153(4):362-9 - PubMed
  20. J Cell Biol. 1985 Sep;101(3):1144-52 - PubMed
  21. Planta. 1983 Aug;158(5):403-9 - PubMed
  22. J Cell Biol. 1972 Sep;54(3):507-39 - PubMed
  23. J Cell Biol. 1980 Mar;84(3):599-617 - PubMed

Publication Types