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Smart CC, Amrhein N. Ultrastructural localisation by protein A-gold immunocytochemistry of 5-enolpyruvylshikimic acid 3-phosphate synthase in a plant cell culture which overproduces the enzyme. Planta. 1987;170(1):1-6doi: 10.1007/BF00392373.
Smart, C. C., & Amrhein, N. (1987). Ultrastructural localisation by protein A-gold immunocytochemistry of 5-enolpyruvylshikimic acid 3-phosphate synthase in a plant cell culture which overproduces the enzyme. Planta, 170(1), 1-6. https://doi.org/10.1007/BF00392373
Smart, C C, and Amrhein, N. "Ultrastructural localisation by protein A-gold immunocytochemistry of 5-enolpyruvylshikimic acid 3-phosphate synthase in a plant cell culture which overproduces the enzyme." Planta vol. 170,1 (1987): 1-6. doi: https://doi.org/10.1007/BF00392373
Smart CC, Amrhein N. Ultrastructural localisation by protein A-gold immunocytochemistry of 5-enolpyruvylshikimic acid 3-phosphate synthase in a plant cell culture which overproduces the enzyme. Planta. 1987 Jan;170(1):1-6. doi: 10.1007/BF00392373. PMID: 24232834.
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Planta. 1987 Jan;170(1):1-6. doi: 10.1007/BF00392373.

Ultrastructural localisation by protein A-gold immunocytochemistry of 5-enolpyruvylshikimic acid 3-phosphate synthase in a plant cell culture which overproduces the enzyme.

Planta

C C Smart, N Amrhein

Affiliations

  1. Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität Bochum, D-4630, Bochum, Federal Republic of Germany.

PMID: 24232834 DOI: 10.1007/BF00392373

Abstract

Recently we have shown that cultured cells of the higher plant Corydalis sempervirens Pers., adapted to growth in the presence of high concentrations of the herbicide glyphosate, a potent specific inhibitor of the shikimate pathway enzyme 5-enolpyruvylshikimic acid 3-phosphate (EPSP) synthase (EC 2.5.1.19, 3-phosphoshikimate 1-carboxyvinyltransferase) oversynthesize the EPSP synthase protein (Smart et al., 1985, J. Biol. Chem. 260, 16338-16346). We now report that the EPSP synthase protein can be detected in cells of the adapted as well as of the non-adapted strain by the use of protein A-colloidal gold immunocytochemistry. The overproduced EPSP synthase in the glyphosate-adapted cells is located exclusively in the plastid and we find no evidence for the existence of extra-plastidic EPSP synthase in either strain.

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