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Coleman GD, Ernst SG. In vitro shoot regeneration of Populus deltoides: effect of cytokinin and genotype. Plant Cell Rep. 1989;8(8):459-62doi: 10.1007/BF00269048.
Coleman, G. D., & Ernst, S. G. (1989). In vitro shoot regeneration of Populus deltoides: effect of cytokinin and genotype. Plant cell reports, 8(8), 459-62. https://doi.org/10.1007/BF00269048
Coleman, G D, and Ernst, S G. "In vitro shoot regeneration of Populus deltoides: effect of cytokinin and genotype." Plant cell reports vol. 8,8 (1989): 459-62. doi: https://doi.org/10.1007/BF00269048
Coleman GD, Ernst SG. In vitro shoot regeneration of Populus deltoides: effect of cytokinin and genotype. Plant Cell Rep. 1989 Dec;8(8):459-62. doi: 10.1007/BF00269048. PMID: 24233528.
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Plant Cell Rep. 1989 Dec;8(8):459-62. doi: 10.1007/BF00269048.

In vitro shoot regeneration of Populus deltoides: effect of cytokinin and genotype.

Plant cell reports

G D Coleman, S G Ernst

Affiliations

  1. Department of Forestry, Fisheries and Wildlife, University of Nebraska-Lincoln, 68583, Lincoln, NE, USA.

PMID: 24233528 DOI: 10.1007/BF00269048

Abstract

Treatment differences were observed in the in vitro adventitious shoot regeneration response from internodal explants of three genotypes of Populus deltoides cultured on media supplemented with five concentrations each of the cytokinins 6-benzyladenine, 2-isopentyladenine, and zeatin. For each of the three genotypes, the greatest number of shoots were consistently regenerated on media containing the cytokinin zeatin. Tissue necrosis resulted when explants from any of the three genotypes were cultured on media supplemented with 6-benzyladenine. A zeatin concentration by genotype interaction was also observed. Genotypic differences in shoot regeneration were observed for 16 genotypes of Populus deltoides when cultured on medium supplemented with 0.5 mgL(-1) zeatin. Six genotypes were highly recalcitrant and failed to regenerate shoots. The percent of explants regenerating was greater than 50% for four genotypes.

References

  1. Tree Physiol. 1989 Jun;5(2):219-27 - PubMed
  2. J Plant Physiol. 1984;116(4):313-21 - PubMed
  3. Theor Appl Genet. 1986 Apr;72(1):3-10 - PubMed

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