Display options
Cite
Akagi H, Nakamura A, Sawada R, et al. Genetic diagnosis of cytoplasmic male sterile cybrid plants of rice. Theor Appl Genet. 1995;90(7):948-51doi: 10.1007/BF00222907.
Akagi, H., Nakamura, A., Sawada, R., Oka, M., & Fujimura, T. (1995). Genetic diagnosis of cytoplasmic male sterile cybrid plants of rice. TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 90(7), 948-51. https://doi.org/10.1007/BF00222907
Akagi, H, et al. "Genetic diagnosis of cytoplasmic male sterile cybrid plants of rice." TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik vol. 90,7 (1995): 948-51. doi: https://doi.org/10.1007/BF00222907
Akagi H, Nakamura A, Sawada R, Oka M, Fujimura T. Genetic diagnosis of cytoplasmic male sterile cybrid plants of rice. Theor Appl Genet. 1995 Jun;90(7):948-51. doi: 10.1007/BF00222907. PMID: 24173048.
Copy Download .nbib Format: NLM
Share it on

Theor Appl Genet. 1995 Jun;90(7):948-51. doi: 10.1007/BF00222907.

Genetic diagnosis of cytoplasmic male sterile cybrid plants of rice.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

H Akagi, A Nakamura, R Sawada, M Oka, T Fujimura

Affiliations

  1. Plant Biotechnology Laboratory, Life Science Institute, Mitsui Toatsu Chemicals Inc., Togo 1144, 297, Mobara, Japan.

PMID: 24173048 DOI: 10.1007/BF00222907

Abstract

Twelve Japanese rice cultivars were converted to CMS by asymmetric protoplast fusion with MTC-5A, the cytoplasm of which was derived from an indica rice, Chinsurah Boro II. With the exception of the cybrids that had a nucleus from Hoshiyutaka, most of these cybrid plants were sterile. The unique sequence downstream from the mitochondrial atp6 of MTC-5A was specifically amplified in the sterile cybrid plants by PCR. All progenies of the cybrid plants carrying this unique sequence were sterile. On the other hand, in some of the sterile cybrid plants in which the unique sequence was not amplified by PCR, fertility was recovered in their progenies. Somaclonal mutation may have caused sterility in these cybrids. Only the cybrid plants that had the unique sequence detected by PCR were CMS. Thus, the CMS plants can be selected rapidly and easily by PCR, at an early stage of plant regeneration. Soon after transplanting the regenerated plants to a green house, fertile cybrids and sterile cybrids produced by somaclonal mutation can be removed. These findings also show that the unique region downstream from atp6 is tightly linked with the CMS phenotype.

References

  1. Curr Genet. 1992 Feb;21(2):153-9 - PubMed
  2. EMBO J. 1993 Apr;12(4):1437-46 - PubMed
  3. Cell. 1987 Jul 3;50(1):41-9 - PubMed
  4. Nucleic Acids Res. 1991 Mar 25;19(6):1349 - PubMed
  5. Plant Cell. 1991 Dec;3(12):1349-62 - PubMed
  6. Curr Genet. 1994 Jan;25(1):52-8 - PubMed
  7. Cell. 1986 Feb 14;44(3):439-49 - PubMed

Publication Types