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Saxena PK, Mii M, Crosby WL, et al. Transplantation of isolated nuclei into plant protoplasts : A novel technique for introducing foreign DNA into plant cells. Planta. 1986;168(1):29-35doi: 10.1007/BF00407005.
Saxena, P. K., Mii, M., Crosby, W. L., Fowke, L. C., & King, J. (1986). Transplantation of isolated nuclei into plant protoplasts : A novel technique for introducing foreign DNA into plant cells. Planta, 168(1), 29-35. https://doi.org/10.1007/BF00407005
Saxena, P K, et al. "Transplantation of isolated nuclei into plant protoplasts : A novel technique for introducing foreign DNA into plant cells." Planta vol. 168,1 (1986): 29-35. doi: https://doi.org/10.1007/BF00407005
Saxena PK, Mii M, Crosby WL, Fowke LC, King J. Transplantation of isolated nuclei into plant protoplasts : A novel technique for introducing foreign DNA into plant cells. Planta. 1986 May;168(1):29-35. doi: 10.1007/BF00407005. PMID: 24233731.
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Planta. 1986 May;168(1):29-35. doi: 10.1007/BF00407005.

Transplantation of isolated nuclei into plant protoplasts : A novel technique for introducing foreign DNA into plant cells.

Planta

P K Saxena, M Mii, W L Crosby, L C Fowke, J King

Affiliations

  1. Department of Biology, University of Saskatchewan, S7N 0W0, Saskatoon, Sask, Canada.

PMID: 24233731 DOI: 10.1007/BF00407005

Abstract

The uptake of isolated nuclei from Vicia hajastana Grossh. cells into protoplasts of an auxotrophic cell line of Datura innoxia P. Mill. was induced under the influence of polyethylene glycol and Ca(2+) at pH 6.8. The frequency of nuclear uptake varied from 0.8 to 2.3% and that of the recovery of prototrophic clones from 10(-5) to 6·10(-4). The prototrophic nuclear fusion products following nuclear uptake could be rescued by initial culture of the protoplasts in non-selective conditions and by the subsequent use of feeder cell layers to support the growth of surviving colonies on a selective medium. The presence of Vicia genomic DNA in some prototrophic clones was confirmed by dot-blot hybridization using Datura and Vicia DNA probes. In certain transformed clones, the recovery of prototrophy was accompanied by the restoration of morphogenetic potential. Welldeveloped shoots typical of wild-type Datura could be regenerated employing an appropriate regeneration medium.

References

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