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Brodelius P, Nilsson K, Mosbach K. Production of α-keto acids Part I. Immobilized cells ofTrigonopsis variabilis containing D-amino acid oxidase. Appl Biochem Biotechnol. 1981;6(4):293-307doi: 10.1007/BF02798280.
Brodelius, P., Nilsson, K., & Mosbach, K. (1981). Production of α-keto acids Part I. Immobilized cells ofTrigonopsis variabilis containing D-amino acid oxidase. Applied biochemistry and biotechnology, 6(4), 293-307. https://doi.org/10.1007/BF02798280
Brodelius, P, et al. "Production of α-keto acids Part I. Immobilized cells ofTrigonopsis variabilis containing D-amino acid oxidase." Applied biochemistry and biotechnology vol. 6,4 (1981): 293-307. doi: https://doi.org/10.1007/BF02798280
Brodelius P, Nilsson K, Mosbach K. Production of α-keto acids Part I. Immobilized cells ofTrigonopsis variabilis containing D-amino acid oxidase. Appl Biochem Biotechnol. 1981 Dec;6(4):293-307. doi: 10.1007/BF02798280. PMID: 24233977.
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Appl Biochem Biotechnol. 1981 Dec;6(4):293-307. doi: 10.1007/BF02798280.

Production of α-keto acids Part I. Immobilized cells ofTrigonopsis variabilis containing D-amino acid oxidase.

Applied biochemistry and biotechnology

P Brodelius, K Nilsson, K Mosbach

Affiliations

  1. Pure and Applied Biochemistry, University of Lund, Chemical Center, PO Box 740, S-22007, Lund, Sweden.

PMID: 24233977 DOI: 10.1007/BF02798280

Abstract

Whole cells ofTrigonopsis variabilis were immobilized by entrapment in Ca(2+)-alginate and used for the production of α-keto acids from the corresponding D-amino acids. The D-amino acid oxidase within the immobilized cells has a broad substrate specificity. Hydrogen peroxide formed in the enzymatic reaction was efficiently hydrolyzed by manganese oxide co-immobilized with the cells. The amino acid oxidase activity was assayed with a new method based on reversed-phase HPLC. Oxygen requirements, bead size, concentration of cells in the beads, flow rate, and other factors were investigated in a " trickle-bed " reactor.

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