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Parker CE, Perkins JR, Tomer KB, et al. Application of nanoscale packed capillary liquid chromatography (75 μm id) and capillary zone electrophoresis/electrospray ionization mass spectrometry to the analysis of macrolide antibiotics. J Am Soc Mass Spectrom. 1992;3(5):563-74doi: 10.1016/1044-0305(92)85034-H.
Parker, C. E., Perkins, J. R., Tomer, K. B., Shida, Y., O'Hara, K., & Kono, M. (1992). Application of nanoscale packed capillary liquid chromatography (75 μm id) and capillary zone electrophoresis/electrospray ionization mass spectrometry to the analysis of macrolide antibiotics. Journal of the American Society for Mass Spectrometry, 3(5), 563-74. https://doi.org/10.1016/1044-0305(92)85034-H
Parker, C E, et al. "Application of nanoscale packed capillary liquid chromatography (75 μm id) and capillary zone electrophoresis/electrospray ionization mass spectrometry to the analysis of macrolide antibiotics." Journal of the American Society for Mass Spectrometry vol. 3,5 (1992): 563-74. doi: https://doi.org/10.1016/1044-0305(92)85034-H
Parker CE, Perkins JR, Tomer KB, Shida Y, O'Hara K, Kono M. Application of nanoscale packed capillary liquid chromatography (75 μm id) and capillary zone electrophoresis/electrospray ionization mass spectrometry to the analysis of macrolide antibiotics. J Am Soc Mass Spectrom. 1992 Jul;3(5):563-74. doi: 10.1016/1044-0305(92)85034-H. PMID: 24234500.
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J Am Soc Mass Spectrom. 1992 Jul;3(5):563-74. doi: 10.1016/1044-0305(92)85034-H.

Application of nanoscale packed capillary liquid chromatography (75 μm id) and capillary zone electrophoresis/electrospray ionization mass spectrometry to the analysis of macrolide antibiotics.

Journal of the American Society for Mass Spectrometry

C E Parker, J R Perkins, K B Tomer, Y Shida, K O'Hara, M Kono

Affiliations

  1. Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, 27709, Research Triangle Park, NC.

PMID: 24234500 DOI: 10.1016/1044-0305(92)85034-H

Abstract

Nanoscale separation techniques, nanoscale packed capillary columns (75 μm id), and capillary zone electrophoresis (CZE), on-line with electrospray mass spectrometry (ESI/MS), were applied to the separation of a series of ten macrolide antibiotics. Both techniques use sub-microliter-per-minute flow rates through the analytical column and therefore require an electrospray probe that incorporates coaxial sheath flow. Positive ion electrospray mass spectra of these compounds yielded mainly protonated molecules. Fragmentation to yield structurally significant fragment ions was achieved by collision-induced dissociation (CID) at increased skimmer voltages. Separations were achieved using both techniques, with CZE/ESI/MS showing improved peak shapes and detection limits combined with faster analysis times. Nanoscale packed capillary columns provided better chromatographic resolution and was less susceptible to peak broadening caused by overloading of the analytes.

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